In vitro nanotoxicity of single-walled carbon nanotube-dendrimer nanocomplexes against murine myoblast cells
In vitro nanotoxicity of single-walled carbon nanotube-dendrimer nanocomplexes against murine myoblast cells
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DOI：
10.1016/j.toxlet.2013.02.009
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发表时间：
2013-05-10
影响因子：
3.5
通讯作者：
Zucolotto, V.
中科院分区：
文献类型：
--
作者：
Cancino, J.;Paino, I. M. M.;Zucolotto, V.
单壁碳纳米管(SWCNTs)和聚酰胺胺树枝状大分子(PAMAM)已被提出用于各种生物医学应用。这两种分子的结合使这种新型复合纳米材料具有高度的功能化，并可用于治疗和药物输送系统。然而，最近的毒理学研究表明，SWCNTs和PAMAM等纳米材料在生物环境中可能具有很高的毒性。为了阐明这种行为，在过去的几年里，人们用不同的培养细胞进行了体外研究。本研究重点研究了SWCNT-PAMAM纳米材料及其单个组分对C2C12小鼠细胞系的影响，C2C12小鼠是干细胞和祖细胞的混合群体。用毒理学分析中常用的不同技术研究了细胞与纳米材料之间的相互作用。结果表明，SWCNT-PAMAM和PAMAM均能抑制C2C12细胞的增殖，并引起细胞DNA损伤。流式细胞仪的数据显示，与其他纳米材料相比，SWCNT对C2C12细胞的毒性较小。结果表明，SWCNT、SWCNT-PAMAM和PAMAM对C2C12细胞的毒性与纳米材料的荷电性密切相关。(C)2013爱思唯尔爱尔兰有限公司。保留所有权利。
Single-wall carbon nanotubes (SWCNTs) and polyamidoamine dendrimers (PAMAM) have been proposed for a variety of biomedical applications. The combination of both molecules makes this new composite nanomaterial highly functionalizable and versatile to theranostic and drug-delivery systems. However, recent toxicological studies have shown that nanomaterials such as SWCNTs and PAMAM may have high toxicity in biological environments. Aiming to elucidate such behavior, in vitro studies with different cultured cells have been conducted in the past few years. This study focuses on the effects of SWCNT-PAMAM nanomaterials and their individual components on the C2C12 murine cell line, which is a mixed population of stem and progenitor cells. The interactions between the cells and the nanomaterials were studied with different techniques usually employed in toxicological analyses. The results showed that SWCNT-PAMAM and PAMAM inhibited the proliferation and caused DNA damage of C2C12 cells. Data from flow cytometry revealed a less toxicity in C2C12 cells exposed to SWCNT compared to the other nanomaterials. The results indicated that the toxicity of SWCNT, SWCNT-PAMAM and PAMAM in C2C12 cells can be strongly correlated with the charge of the nanomaterials. (C) 2013 Elsevier Ireland Ltd. All rights reserved.