The behavioral and physiological effects of high-fat diet and alcohol consumption: Sex differences in C57BL6/J mice.
The behavioral and physiological effects of high-fat diet and alcohol consumption: Sex differences in C57BL6/J mice.
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DOI：
10.1002/brb3.708
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发表时间：
2017-06
影响因子：
3.1
通讯作者：
Seggio JA
中科院分区：
文献类型：
--
作者：
Gelineau RR;Arruda NL;Hicks JA;Monteiro De Pina I;Hatzidis A;Seggio JA
动物研究可以是一个很好的工具，以各种不同的方式调查性别差异，包括对药物治疗的行为和生理反应以及不同的“生活方式变量”，如饮食。众所周知，高脂肪饮食和酒精的消费会影响焦虑行为和整体健康。该项目研究了高脂饮食和酒精摄入及其组合如何影响雄性和雌性C57 BL/6 J小鼠的行为和生理。将小鼠分为三个食物组：高脂肪饮食、10%脂肪饮食和常规食物，每组与水或10%酒精配对。行为测定包括饮食和酒精偏好、明暗箱、旷场以及进食和饮水测量。生理指标包括葡萄糖耐量试验和脑源性神经营养因子、胰岛素和瘦素水平的测量。雌性和雄性在开放领域有所不同，因为雄性小鼠减少活动，而雌性小鼠在食用高脂肪饮食时增加活动。虽然女性比男性消耗更多的乙醇，但酒精消费能够改善葡萄糖耐量并增加男女的焦虑。最后，女性对高脂饮食引起的生理变化的抵抗力高于男性，因为与食用高脂饮食的男性相比，食用高脂饮食的女性表现出胰岛素分泌减少，脑源性神经营养因子水平变化较小，葡萄糖耐量更好。这些结果表明，对高脂肪饮食和饮酒的反应是性别依赖的，与女性相比，男性在行为和生理上受到高脂肪饮食的影响更大。
Animal studies can be a great tool to investigate sex differences in a variety of different ways, including behavioral and physiological responses to drug treatments and different “lifestyle variables” such as diets. Consumption of both high‐fat diets and alcohol is known to affect anxiety behaviors and overall health. This project investigated how high‐fat diet and alcohol access and its combination affected the behavior and physiology of male and female C57BL/6J mice. Mice were separated into three food groups: high‐fat diet, 10% fat diet, and regular chow, and each group was paired with either water or 10% alcohol. Behavioral assays included diet and alcohol preference, light‐dark box, open field, and feeding and drinking measurements. Physiological measures included glucose tolerance tests and measurement of brain‐derived neurotrophic factor, insulin, and leptin levels. Females and males differed in the open field, as male mice decreased activity, while females increased activity when consuming high‐fat diet. While females consumed more ethanol than males, alcohol consumption was able to improve glucose tolerance and increase anxiety in both sexes. Lastly, females were more resistant to the physiological changes caused by high‐fat diet than males, as females consuming high‐fat diet exhibited decreased insulin secretion, less change to brain‐derived neurotrophic factor levels, and better glucose tolerance than males consuming high‐fat diet. These results suggest that the response to high‐fat diet and alcohol consumption is sex dependent and that males are more affected both behaviorally and physiologically by high‐fat diet compared to females.