Decreased [3H]nitrendipine binding in the brainstem of deoxycorticosterone-NaCl hypertensive rats.
Decreased [3H]nitrendipine binding in the brainstem of deoxycorticosterone-NaCl hypertensive rats.
复制标题
去氧皮质酮-氯化钠高血压大鼠脑干中[3H]尼群地平结合减少。
DOI：
10.1016/0024-3205(85)90534-x
复制
发表时间：
1985
期刊：
影响因子：
6.1
通讯作者：
Roeske,WR
中科院分区：
文献类型：
--
作者：
Lee,HR;Watson,M;Yamamura,HI;Roeske,WR
在单侧肾切除的脱氧皮质酮（DOCA）-NaCl高血压大鼠和溶剂处理的正常血压对照同窝仔中进行了1，4-二氢吡啶钙通道拮抗剂[3H]尼群地平（[3H] NTD）的结合研究。治疗6周后，与血压正常（135 mmHg）的对照组相比，高血压（199 mmHg，动脉收缩压）DOCA大鼠的心脏、左心室和肾脏重量显著增加。与对照组（116 ± 24 fmol/mg蛋白）相比，DOCA-NaCl大鼠脑干中的[3H] NTD结合显著降低（51 ± 5 fmol/mg蛋白）。然而，DOCA-NaCl（0.43 ± 0.03 nM）或对照大鼠（0.62 ± 0.06 nM）的[3H] NTD解离常数无显著差异。大脑皮质和左心室组织受体结合密度或亲和力无显着变化。特异性[3H] NTD结合在其他选定的脑区或心房中没有显著改变。这些数据表明，与脑干钙通道相关的二氢吡啶结合位点的改变可能参与DOCA-NaCl诱导的高血压的病因学。
Binding studies with the 1,4 dihydropyridine calcium channel antagonist [3H]nitrendipine ([3H]NTD) were performed in uninephrectomized, deoxycorticosterone (DOCA)-NaCl hypertensive rats and vehicle treated normotensive control littermates. After 6 weeks of treatment, hypertensive (199 mmHg, systolic arterial pressure) DOCA rats showed significantly increased heart, left ventricle, and kidney weight in contrast to normotensive (135 mmHg) controls. [3H]NTD binding in the brainstem was significantly reduced (51 ± 5 fmol/mg protein) in DOCA-NaCl rats, as compared to controls (116 ± 24 fmol/mg protein). However, no significant differences were found in the [3H]NTD dissociation constants for DOCA-NaCl (0.43 ± 0.03 nM) or control rats (0.62 ± 0.06 nM). Cerebral cortical and left ventricular tissue showed no significant alterations in receptor binding density or affinity. Specific [3H]NTD binding was not significantly altered in other selected brain regions or the atria. These data suggest that alterations in the dihydropyridine binding sites associated with calcium channels in the brainstem may be involved in the etiology of DOCA-NaCl-induced hypertension.