Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B1, ochratoxin A and zearalenone in agro-food
Multi-component immunochromatographic assay for simultaneous detection of aflatoxin B1, ochratoxin A and zearalenone in agro-food
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多组分免疫色谱法同时检测农产品中黄曲霉毒素B1、赭曲霉毒素A和玉米赤霉烯酮
DOI：
10.1016/j.bios.2013.05.039
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发表时间：
2013-11-15
影响因子：
12.6
通讯作者：
Tang, Xiaoqian
中科院分区：
文献类型：
--
作者：
Li, Xin;Li, Peiwu;Tang, Xiaoqian
真菌毒素是一种毒性很强的污染物，对人类和动物的健康造成了严重的威胁。黄曲霉毒素B-1（AFB（1））、赭曲霉毒素A（OTA）和玉米赤霉烯酮（ZEA）通常存在于食物和饲料中。建立了一种同时快速检测农产品中3种真菌毒素的多组分免疫层析方法。该策略是基于抗体-胶体金纳米颗粒结合物探针与真菌毒素或真菌毒素抗原之间的竞争性免疫反应进行的。各单克隆抗体特异性识别其对应的毒素和抗原，且无交叉反应。将三种真菌毒素抗原固定在硝酸纤维素膜反应区的三条测试线上，这使得能够在一次测试中同时检测。该试纸条对AFB（1）、OTA和ZEA的目视检测限分别为0.25 ng/mL、0.5 ng/mL和1 ng/mL。使用加标和自然污染的花生，玉米和大米样品的测定进行了评估。结果与酶联免疫吸附试验结果一致。该伊卡可为农产品中多种真菌毒素的现场检测提供一种快速有效的方法。皇冠版权所有（C）2013由爱思唯尔B. V.出版保留所有权利。
Mycotoxins are highly toxic contaminants and have induced health threat to human and animals. Aflatoxin B-1 (AFB(1)), ochratoxin A (OTA) and zearalenone (ZEA) commonly occur in food and feed. A multi-component immunochromatographic assay (ICA) was developed for rapid and simultaneous determination of these three mycotoxins in agro-food. The strategy was performed based on the competitive immunoreactions between antibody-colloidal gold nanoparticle conjugate probes and mycotoxins or mycotixin antigens. Each monoclonal antibody specially recognize its corresponding mycotoxin and antigen, and there was no cross reactivity in the assay. Three mycotixin antigens were immobilized as three test lines in the nitrocellulose membrane reaction zone, which enable the simultaneous detection in one single test. The visible ICA results were obtained in 20 min. The visual detection limits of this strip test for the AFB(1), OTA and ZEA were 025 ng/mL, 0.5 ng/mL and 1 ng/mL, respectively. The assay was evaluated using spiked and naturally contaminated peanuts, maize and rice samples. The results were in accordance with those obtained using enzyme-linked immunosorbent assay. In summary, this developed ICA could provide an effective and rapid approach for onsite detection of multi-mycotoxin in agro-food samples without any expensive instrument. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.