Developmental Validation of a novel 5 dye Y-STR System comprising the 27 YfilerPlus loci.
Developmental Validation of a novel 5 dye Y-STR System comprising the 27 YfilerPlus loci.
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包含 27 个 YfilerPlus 位点的新型 5 染料 Y-STR 系统的开发验证
DOI：
10.1038/srep29557
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发表时间：
2016-07-13
影响因子：
4.6
通讯作者：
Ma S
中科院分区：
文献类型：
--
作者：
Bai R;Liu Y;Li Z;Jin H;Tian Q;Shi M;Ma S
在这项研究中，一个新的STRtyper-27系统，包括相同的Yfiler Plus基因座，（DYS19、DYS389 I、DYS389 II、DYS390、DYS391、DYS392、DYS393、DYS385 a/B、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635、Y-GATA H4、DYS449、DYS460、DYS481、DYS518、DYS533、DYS456、DYS458、DYS635、Y-GATA H4、DYS449、DYS460、DYS481、DYS518、DYS533，DYS 570、DYS 576、DYS 627和DYF 387 S1 a/B）。引物、内部大小标准品、等位基因梯和基质标准品组在内部设计并创建用于该多重系统。本文介绍了使用STR typer-27 Y系统进行的验证研究，使用3130 XL遗传分析仪进行片段长度检测，包括以下参数和方面的分析：灵敏度，种属特异性，抑制，单倍型一致性，精密度，口吃，DNA混合物和稳定性研究与犯罪现场样本。研究表明，STRtyper-27 Y系统提供了与最新的Yfiler Plus试剂盒相当的整体性能，但在仪器平台和软件方面具有增强的兼容性，允许法医实验室在自己的5染料Y-STR化学系统/环境中进行法医应用并评估其性能，而无需软件或仪器升级。
In this study, a new STRtyper-27 system, including the same Yfiler Plus loci (DYS19, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS385a/b, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS635, Y-GATA H4, DYS449, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS627 and DYF387S1a/b), was established using a set of 5 fluorescent dye labels. Primers, internal size standard, allelic ladders and matrix standard set were designed and created in-house for this multiplex system. This paper describes the validation studies conducted with the STRtyper-27Y system using a 3130XL genetic analyzer for fragment length detection that included the analysis of the following parameters and aspects: sensitivity, species specificity, inhibition, haplotype concordance, precision, stutter, DNA mixtures, and stability studies with crime scene samples. The studies demonstrated, that the STRtyper-27Y system provided equivalent overall performance comparable to the latest Yfiler Plus kit, but with enhanced compatibility in terms of instrument platforms and software allowing forensic laboratories to conduct its forensic application and evaluate its performance, all in their own 5 dye Y-STR chemistry system /environment without software or instrument upgrades.