Molecular analysis of the INCENPs (inner centromere proteins): separate domains are required for association with microtubules during interphase and with the central spindle during anaphase.
Molecular analysis of the INCENPs (inner centromere proteins): separate domains are required for association with microtubules during interphase and with the central spindle during anaphase.
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对incenps的分子分析（内侧丝粒蛋白）：在相间期间与微管缔合的单独结构域是需要单独的结构域，并且与后期期间的中央纺锤体相关。
DOI：
10.1083/jcb.123.2.373
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发表时间：
1993-10
影响因子：
7.8
通讯作者：
Earnshaw, W C
中科院分区：
文献类型：
--
作者：
Mackay, A M;Eckley, D M;Chue, C;Earnshaw, W C
最近有人提出，有丝分裂染色体将某些细胞骨架蛋白转运至中期板，使得这些蛋白能够随后参与后期纺锤体和卵裂沟的组装。为了了解这些蛋白质如何实现其双重染色体：细胞骨架作用，我们开始对内部着丝粒蛋白（INCENP）（“染色体过客蛋白”类别的创始人成员）进行分子和功能分析。回收了编码两种鸡 INCENP 开放阅读框的 cDNA 克隆。预测的蛋白质 I 类 INCENP (96,357 D) 和 II 类 INCENP (100,931 D) 是新颖的，并且由于在 II 类编码区内包含 38 个密码子插入而彼此不同。鸡 INCENP 在哺乳动物细胞中的瞬时表达证实，这些蛋白质从染色体转移到细胞骨架所需的信号和结构在进化上是保守的。此外，这些研究表明 INCENP 与细胞骨架的关联是复杂的。氨基末端42个氨基酸残基是INCENP从染色体转移到有丝分裂纺锤体在后期的必需的，但不是INCENP与细胞质微管的结合所必需的。相比之下，内部 200 个氨基酸的卷曲螺旋结构域对于与微管的关联是必需的，但对于纺锤体的关联来说是可有可无的。这些实验表明，在有丝分裂期间从后期开始组装特殊细胞骨架结构所需的蛋白质可能在整个间期被隔离在细胞核中，以防止它们破坏间期细胞骨架，并确保它们在有丝分裂期间的正确定位。
It has recently been proposed that mitotic chromosomes transport certain cytoskeletal proteins to the metaphase plate so that these proteins are able to subsequently participate in the assembly of the anaphase spindle and the cleavage furrow. To understand how such proteins accomplish their dual chromosomal: cytoskeletal role, we have begun a molecular and functional analysis of the inner centromere proteins (INCENPs), founder members of the class of "chromosome passenger proteins". cDNA clones encoding the open reading frames of the two chicken INCENPs were recovered. The predicted proteins, class I INCENP (96,357 D) and class II INCENP (100,931 D) are novel, and differ from each other by the inclusion of a 38-codon insert within the class II coding region. Transient expression of the chicken INCENPs in mammalian cells confirms that the signals and structures required for the transfer of these proteins from chromosomes to cytoskeleton are evolutionarily conserved. Furthermore, these studies reveal that INCENP association with the cytoskeleton is complex. The amino-terminal 42- amino acid residues are required for transfer of the INCENPs from the chromosomes to the mitotic spindle at anaphase, but not for binding of INCENPs to cytoplasmic microtubules. In contrast, an internal 200 amino acid coiled-coil domain was required for association with microtubules, but dispensable for spindle association. These experiments suggest that proteins required for assembly of specialized cytoskeletal structures during mitosis from anaphase onwards might be sequestered in the nucleus throughout interphase to keep them from disrupting the interphase cytoskeleton, and to ensure their correct positioning during mitosis.