Direct cell entry of gold/iron-oxide magnetic nanoparticles in adenovirus mediated gene delivery
Direct cell entry of gold/iron-oxide magnetic nanoparticles in adenovirus mediated gene delivery
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DOI：
10.1016/j.biomaterials.2008.12.015
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发表时间：
2009-03-01
期刊：
影响因子：
14
通讯作者：
Nakagawa, Shinsaku
中科院分区：
文献类型：
--
作者：
Kamei, Kazumasa;Mukai, Yohei;Nakagawa, Shinsaku
金/氧化铁磁性纳米粒子 (GoldMAN) 赋予各种生物分子有用的磁性。固定在磁性纳米颗粒表面的金纳米颗粒可以通过 Au-S 键与生物分子结合。在这里，我们展示了利用 GoldMAN 和磁场诱导细胞内转导的实际应用。该方法对于将广泛用于体外/体内基因转移的腺病毒基因传递载体（Ad）应用于Ad抗性细胞具有巨大的潜力。我们证明，Ad 很容易固定在 GoldMAN 上，并且 Ad/GoldMAN 复合物通过磁场引入细胞中，这使基因表达量比单独的 Ad 增加了 1000 倍以上。 GoldMAN 直接穿透质膜，不依赖于细胞表面病毒受体和内吞途径。该机制将有助于提高Ad的基因表达效率。该技术是扩展Ad向性和提高转导效率的有用工具。由于其有趣的细胞进入机制，GoldMAN 还使得细胞内各种生物分子的有效利用成为可能。 (C) 2008 Elsevier Ltd. 保留所有权利。
Gold/iron-oxide MAgnetic Nanoparticles (GoldMAN) imparts useful magnetic properties to various biomolecules. Gold nanoparticles immobilized on the surface of magnetic nanoparticles allow for the conjugation of biomolecules via an Au-S bond. Here, we present a practical application by utilizing GoldMAN and a magnetic field to induce intracellular transduction. This method has great potential for application of the adenovirus gene delivery vector (Ad), widely used for in vitro/in vivo gene transfer, to Ad-resistant cells. We demonstrated that Ad was easily immobilized on GoldMAN and the Ad/GoldMAN complex was introduced into the cell by the magnetic field, which increased gene expression over 1000 times that of Ad alone. The GoldMAN penetrated the plasma membrane directly, independent of the cell-surface virus receptors and endocytosis pathway. This mechanism will contribute to improve the gene expression efficiency of Ad. This technology is a useful tool for extending Ad tropism and enhancing transduction efficiency. GoldMAN also makes possible the effective use of various biomolecules within the cell because of its interesting cell-entry mechanism. (C) 2008 Elsevier Ltd. All rights reserved.