Fate of intracellular H2S/HS- and metallo-proteins.
Fate of intracellular H2S/HS- and metallo-proteins.
复制标题
细胞内 H2S/HS 和金属蛋白的命运。
DOI：
10.1016/j.resp.2013.05.029
复制
发表时间：
2013
影响因子：
2.3
通讯作者：
Klingerman,CandiceM
中科院分区：
文献类型：
--
作者：
Haouzi,Philippe;Klingerman,CandiceM
Kenneth Olson最近开发了一种理论模型来预测内源性产生的H2S细胞内分子如何在细胞内外扩散（Olson，2013）。澄清这个问题是主要的兴趣，因为细胞内的H2S，这是主要存在的形式下，其硫酸根阴离子HS-，已被假设是一个重要的演员参与转导的反应缺氧（Olson，2011 a）. Olson模型的主要含义之一，其表明内源产生的H2S在细胞质外的扩散很少（如果有的话），支持这种气体生理作用的研究，基于其在细胞外环境中的测定-体内实验的血液或组织或细胞净化的"浴"-应该以高度的怀疑态度考虑。这一概念证实了先前研究的结果（Furne等人，2008; Whitfield等人，2008），其中确定了妨碍细胞外环境中H2S/HS −准确测定的主要方法学缺陷，解释了文献中报告的血液和组织中硫化物的不切实际的高（μ M）基线水平。尽管正在尝试测量/可视化细胞内H2S/HS-（Lin等人，2013年），理论模型，如奥尔森（奥尔森，2013年）提出的一个，代表了一个合理的参考框架，旨在预测内源性或外源性H2S的命运发展的重要一步。
Kenneth Olson has recently developed a theoretical model to predict how endogenouslygenerated intracellular molecules of H2S would diffuse within and outside the cells (Olson, 2013). Clarifying this question is of major interest since intracellular H2S, which is mostly present under the form of its sulfhydric anion HS−, has been hypothesized to be an important actor involved in the transduction of the response to hypoxia (Olson, 2011a).One of the major implications of Olson’s model, which suggests little, if any, diffusion outside the cytoplasm of endogenously-generated H2S, is that studies supporting a physiological role for this gas, based on its determination in the extracellular milieu-blood for in-vivo experiments or “bath” for tissular or cellular preparations-should be considered with a high degree of skepticism. This notion corroborates results from previous studies (Furne et al., 2008; Whitfield et al., 2008) wherein major methodological pitfalls preventing accurate determination of H2S/HS− in the extracellular milieu were identified, accounting for the unrealistic high (microM) baseline levels of sulfide in the blood and in tissues reported in the literature. Although attempts are being made to measure/visualize intracellular H2S/HS−(Lin et al., 2013), theoretical models, such as the one proposed by Olson (Olson, 2013), represent an essential step in the development of a rational frame of reference aimed at predicting the fate of endogenous–or exogenous-H2S.