Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori
Characterization and functional analysis of serpin-28 gene from silkworm, Bombyx mori
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DOI：
10.1016/j.jip.2018.10.013
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Guoqing Wei
中科院分区：
文献类型：
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作者：
Qiuping Gao;Guoqing Wei
Serine protease inhibitors (Serpins) are a broadly distributed superfamily of proteins with a SERPIN domain and participate in several immune responses. In this study, a serpin-28 gene was identified from silkworm, Bombyx mori. This gene has an open reading frame of 1065 bp that encodes a 354-amino acid residues polypeptide containing one SERPIN domain with a predicted molecular weight of 40.3 kDa. The recombinant Bmserpin-28 protein was expressed in Escherichia coli and the purified protein was used to prepare rabbit anti-Bmserpin-28 polyclonal antibodies. Quantitative real-time PCR analysis revealed that Bmserpin-28 was expressed in all examined tissue, the greatest expression level of Bmserpin-28 was recorded in the fat body and silk gland. The expression pattern of different developmental stages showed that Bmserpin-28 expression level was highest in the pupae, while the lowest expression level was recorded at the egg stage. After challenge with four different microorganisms (Escherichia coli, Beauveria bassiana, Micrococcus luteus and B. mori nuclear polyhedrosis virus), the expression pattern of Bmserpin-28 was investigated in fat body and haemocyte samples. A substantial upregulation of Bmserpin-28 expression level was recorded following pathogen challenge in both the tested tissues. Furthermore, the knockdown of Bmserpin-28 resulted in significant upregulation of antimicrobial peptide genes. In summary, our results indicated that Bmserpin-28 is involved in the innate immunity of Bombyx mori.
