EprS, an autotransporter serine protease, plays an important role in various pathogenic phenotypes of Pseudomonas aeruginosa
EprS, an autotransporter serine protease, plays an important role in various pathogenic phenotypes of Pseudomonas aeruginosa
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EprS是一种自转运丝氨酸蛋白酶，在铜绿假单胞菌的多种致病表型中发挥重要作用
作者：
Kida;Y.;Taira;J.;Kuwano;K.
铜绿假单胞菌具有细胞相关的（鞭毛、皮利、藻酸盐等）和细胞外基质（细胞外基质）。和细胞外（外毒素A、蛋白酶、III型分泌效应物等）毒力因子其中，分泌性蛋白酶对宿主组织的损伤被认为在P.呼吸道感染我们以前报道了一种自转运蛋白酶EprS。铜绿假单胞菌通过蛋白酶激活受体诱导宿主炎症反应。然而，很少有人知道的作用，EprS作为毒力因子的P。铜绿。本研究旨在探讨EprS是否参与P.铜绿假单胞菌，我们的特点是各种致病表型的野生型PAO1菌株和itseprS破坏突变体。在以BSA为唯一碳源和氮源的基本培养基中，eprS突变株的生长速度略低于野生型菌株。因此，这些结果表明，prS将在P的生长中发挥作用。在有限的营养物存在下，例如含有蛋白质材料作为唯一营养源的培养基中，可以使用aerodecosaic。此外，破坏ofeprS导致弹性蛋白酶，色素，自诱导物和表面活性剂的生产减少，游泳和群集运动减少。此外，与野生型菌株相比，eprS突变株与A549细胞结合的能力降低，在白细胞减少小鼠中的毒力减弱。总的来说，这些结果表明，EPRS发挥多效性作用的各种致病表型的P。铜绿。
Pseudomonas aeruginosapossesses an arsenal of both cell-associated (flagella, pili, alginate, etc.) and extracellular (exotoxin A, proteases, type III secretion effectors, etc.) virulence factors. Among them, secreted proteases that damage host tissues are considered to play an important role in the pathogenesis ofP. aeruginosainfections. We previously reported that EprS, an autotransporter protease ofP. aeruginosa, induces host inflammatory responses through protease-activated receptors. However, little is known about the role of EprS as a virulence factor ofP. aeruginosa. In this study, to investigate whether EprS participates in the pathogenicity ofP. aeruginosa, we characterized various pathogenic phenotypes of the wild-type PAO1 strain and itseprS-disrupted mutant. The growth assays demonstrated that the growth of theeprSmutant was somewhat lower than that of the wild-type strain in a minimal medium containing BSA as the sole carbon and nitrogen source. Thus, these results indicate thateprSwould have a role in the growth ofP. aeruginosain the presence of limited nutrients, such as a medium containing proteinaceous materials as a sole nutrient source. Furthermore, disruption ofeprSresulted in a decreased production of elastase, pigments, autoinducers and surfactants, and a reduction of swimming and swarming motilities. In addition, theeprSmutant exhibited a reduction in the ability to associate with A549 cells and an attenuation of virulence in leucopenic mice as compared with the wild-type strain. Collectively, these results suggest that EprS exerts pleiotropic effects on various pathogenic phenotypes ofP. aeruginosa.