Chronic morphine treatment selectively suppresses macrophage colony formation in bone marrow.
Chronic morphine treatment selectively suppresses macrophage colony formation in bone marrow.
复制标题
长期吗啡治疗选择性抑制骨髓中巨噬细胞集落的形成。
DOI：
10.1016/0014-2999(91)90476-7
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发表时间：
1991
影响因子：
5
通讯作者：
Lee,NM
中科院分区：
文献类型：
--
作者：
Roy,S;Ramakrishnan,S;Loh,HH;Lee,NM
阿片类药物在体内和体外对免疫系统有不同的影响，但对其对未成熟祖细胞的相互作用研究较少。我们观察了慢性吗啡处理对小鼠骨髓细胞集落形成的影响。小鼠骨髓细胞植入吗啡颗粒72 h后，对巨噬细胞集落刺激因子(M-CSF)的反应性降低65%。相比之下，慢性吗啡治疗对骨髓细胞对粒细胞/巨噬细胞集落刺激因子(GM-CSF)的反应无影响。从小鼠体内移除吗啡微丸后，巨噬细胞集落形成的抑制作用随时间而被逆转，同时给予纳洛酮和吗啡微丸可完全阻断这种抑制作用。在单次急性剂量吗啡处理的小鼠骨髓细胞中没有观察到集落形成的抑制。未处理的小鼠骨髓细胞与体外吗啡浓度低至25μM孵育7天也可减少巨噬细胞集落形成，阿片肽β-内啡肽作用更强，在低至0.25μM时可显著减少巨噬细胞集落形成。与体内实验结果一致，两种阿片类药物在体外对粒/巨噬细胞集落形成均无显著影响。这些结果表明，阿片类药物可能显著改变免疫细胞的成熟，从而可能对整体免疫能力产生强有力的影响。
Opioids have been shown to have diverse effects on the immune system, both in vivo and in vitro, but their interactions on immature progenitor cells have been little studied. We have examined the effects of chronic morphine treatment of mice on colony formation by bone marrow cells in vitro. Bone marrow cells from mice implanted with morphine pellets for 72 h showed a 65% decrease in their response to macrophage colony stimulating factor (M-CSF). In contrast, chronic morphine treatment had no effect on the response of bone marrow cells to granulocyte/macrophage colony stimulating factor (GM-CSF). Removal of the morphine pellets from the mice resulted in a time-dependent reversal of the inhibition of macrophage colony formation, and the inhibition was completely blocked by simultaneous administration of naloxone and morphine pellets to the mice. No inhibition of colony formation was observed in bone marrow cells from mice treated with a single acute dose of morphine. Incubation of bone marrow cells from untreated mice for 7 days with in vitro morphine concentrations as low as 25 μM also reduced macrophage colony formation, and the opioid peptide β-endorphin was even more potent, significantly reducing macrophage colony formation at concentrations as low as 0.25 μM. In agreement with the in vivo effects, neither opioid in vitro had a significant effect on granuiocyte/macrophage colony formation. These results suggest that opioids may significantly alter the maturation of immune cells, which could result in potent effects on overall immune competence.