Anatomical and Electrophysiological Comparison of CA1 Pyramidal Neurons of the Rat and Mouse
Anatomical and Electrophysiological Comparison of CA1 Pyramidal Neurons of the Rat and Mouse
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DOI：
10.1152/jn.00082.2009
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发表时间：
2009-10-01
影响因子：
2.5
通讯作者：
Chitwood, Raymond A.
中科院分区：
文献类型：
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作者：
Routh, Brandy N.;Johnston, Daniel;Chitwood, Raymond A.
Routh BN，约翰斯顿D，Harris K，Chitwood RA.大鼠和小鼠海马CA 1区锥体神经元的解剖和电生理比较。J Neurophysiol 102：2288-2302，2009.首次发表于2009年8月12日; doi：10.1152/jn.00082.2009。单神经元水平的学习和记忆研究依赖于许多动物模型的使用，最明显的是啮齿动物。虽然许多生理学和解剖学研究已经在大鼠中进行，但基因工程小鼠的出现使得有必要将小鼠中的新结果与大鼠的既定结果进行比较。在这里，我们比较基本的生理和形态学特性，并建立三维房室模型，确定海马CA 1区锥体神经元的一个品系的大鼠，Sprague-Dawley，和两个品系的小鼠，C57 BL/6和129/SvEv。我们报告了三个动物组之间的神经元生理学和解剖学的几个差异，最值得注意的是，129/SvEv小鼠的神经元，但不是C57 BL/6小鼠，具有较高的输入电阻，较低的树突表面积，和较小的棘比大鼠。一个令人惊讶的物种特异性差异的膜共振表明，这两种小鼠品系具有较低水平的超极化激活的非特异性阳离子电流I-h。模拟表明，I-H动力学的差异，而不是最大电导占较低的共振。我们的研究结果表明，在菌株或物种之间获得的数据的比较将需要考虑这些和潜在的其他生理和解剖差异。
Routh BN, Johnston D, Harris K, Chitwood RA. Anatomical and electrophysiological comparison of CA1 pyramidal neurons of the rat and mouse. J Neurophysiol 102: 2288-2302, 2009. First published August 12, 2009; doi: 10.1152/jn.00082.2009. The study of learning and memory at the single-neuron level has relied on the use of many animal models, most notably rodents. Although many physiological and anatomical studies have been carried out in rats, the advent of genetically engineered mice has necessitated the comparison of new results in mice to established results from rats. Here we compare fundamental physiological and morphological properties and create three-dimensional compartmental models of identified hippocampal CA1 pyramidal neurons of one strain of rat, Sprague-Dawley, and two strains of mice, C57BL/6 and 129/SvEv. We report several differences in neuronal physiology and anatomy among the three animal groups, the most notable being that neurons of the 129/SvEv mice, but not the C57BL/6 mice, have higher input resistance, lower dendritic surface area, and smaller spines than those of rats. A surprising species-specific difference in membrane resonance indicates that both mouse strains have lower levels of the hyperpolarization- activated nonspecific cation current I-h. Simulations suggest that differences in I-h kinetics rather than maximal conductance account for the lower resonance. Our findings indicate that comparisons of data obtained across strains or species will need to account for these and potentially other physiological and anatomical differences.