Analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus F and G
Analysis of rotavirus species diversity and evolution including the newly determined full-length genome sequences of rotavirus F and G
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DOI：
10.1016/j.meegid.2012.11.015
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发表时间：
2013-03-01
影响因子：
3.2
通讯作者：
Johne, Reimar
中科院分区：
文献类型：
--
作者：
Kindler, Eveline;Trojnar, Eva;Johne, Reimar
轮状病毒是人类和动物病毒性急性胃肠炎的主要原因。基于VP 6基因的抗原性和核苷酸序列同一性，已经定义了八种不同的轮状病毒种（A-H）。在此，描述了轮状病毒F（株03 V0568）和G（株03 V0567）的第一个完整基因组序列，其长度分别为18，341和18，186 bp。两种病毒都具有位于11个基因组区段的轮状病毒蛋白VP 1至VP 7和NSP 1至NSP 5的开放阅读框。轮状病毒F与其他轮状病毒的核苷酸序列同一性在29.8%（NSP 1基因）和61.7%（VPI基因）之间，轮状病毒G在29.3%（NSP 1 -2基因）和65.9%（NSP 2基因）之间，从而证实它们被分类为单独的病毒种。编码的蛋白质显示显着的序列差异轮状病毒物种。相反，基因组区段的非编码5 '末端序列在所有轮状病毒物种中高度保守。在轮状病毒A/D/F、轮状病毒C和轮状病毒B/G/H之间发现不同的3 '端共有序列。系统发育分析表明轮状病毒分为两个主要分支，即轮状病毒A/C/D/F和轮状病毒B/G/H。在这些分支中，轮状病毒F主要与轮状病毒D聚簇，轮状病毒G与轮状病毒B聚簇。此外，哺乳动物和禽类轮状病毒A株之间的分化，轮状病毒B和C的宿主特异性进化以及禽类轮状病毒A和D之间的古老重配事件的系统发育数据表明。这些结果强调了轮状病毒的高度多样性，作为一个复杂的进化历史的结果。(C)2012 Elsevier B. V.保留所有权利。
Rotaviruses are a leading cause of viral acute gastroenteritis in humans and animals. Eight different rotavirus species (A-H) have been defined based on antigenicity and nucleotide sequence identities of the VP6 gene. Here, the first complete genome sequences of rotavirus F (strain 03V0568) and G (strain 03V0567) with lengths of 18,341 and 18,186 bp, respectively, are described. Both viruses have open reading frames for rotavirus proteins VP1 to VP7 and NSP1 to NSP5 located at the 11 genome segments. Nucleotide sequence identities to other rotaviruses ranged between 29.8% (NSP1 gene) and 61.7% (VPI gene) for rotavirus F and between 29.3% (NSP1-2 gene) and 65.9% (NSP2 gene) for rotavirus G, thus confirming their classification as separate virus species. Encoded proteins revealed remarkable sequence differences among the rotavirus species. In contrast, the non-coding 5'-terminal sequences of the genome segments are highly conserved among all rotavirus species. Different 3'-terminal consensus sequences are found between rotavirus A/D/F, rotavirus C and rotavirus B/G/H. Phylogenetic analyses indicated a separation of rotaviruses in two major clades consisting of rotavirus A/C/D/F and rotavirus B/G/H. Within these clades, rotavirus F mainly clustered with rotavirus D and rotavirus G with rotavirus B. In addition, differentiation among mammalian and avian rotavirus A strains, host-specific evolution of rotavirus B and C as well as an ancient reassortment event between avian rotavirus A and D are indicated by the phylogenetic data. These results underline the high diversity of rotaviruses as a result of a complex evolutionary history. (C) 2012 Elsevier B.V. All rights reserved.