Requirement for 24(S),25-epoxycholesterol for the viability of cultured fibroblasts.
Requirement for 24(S),25-epoxycholesterol for the viability of cultured fibroblasts.
复制标题
培养的成纤维细胞的活力需要 24(S),25-环氧胆固醇。
DOI：
10.1016/0014-4827(87)90180-7
复制
发表时间：
1987
影响因子：
3.7
通讯作者：
Spencer,TA
中科院分区：
文献类型：
--
作者：
Sinensky,M;Logel,J;Phirwa,S;Gayen,AK;Spencer,TA
以前的研究表明，体细胞突变体甲羟戊酸（Mev-1）营养缺陷型，这些细胞迅速失去活力时，剥夺甲羟戊酸补充血清胆固醇的培养基。对培养的哺乳动物细胞中甲羟戊酸代谢的所有已知终产物进行了检测，以确定甲羟戊酸需求的基础。已经发现，最近发现的甲羟戊酸代谢物24（S），25-环氧胆固醇使缺乏甲羟戊酸的Mev-1细胞的生存力部分恢复，而其它结构相似的氧固醇则不能。24（S），25-环氧胆固醇在CHO-K1细胞中似乎具有特异性的重要细胞功能。
Previous studies on a somatic cell mutant auxotrophic for mevalonate (Mev-1) have shown that these cells rapidly lose viability when deprived of mevalonic acid in culture medium supplemented with serum cholesterol. Testing of all known end products of mevalonate metabolism in cultured mammalian cells has been conducted to determine the basis for this mevalonate requirement. It has been found that the recently discovered mevalonate metabolite 24(S),25-epoxycholesterol produces a partial restoration of viability of Mev-1 cells starved for mevalonate, whereas other structurally similar oxysterols do not. It appears that 24(S),25-epoxycholesterol has a specific, vital cellular function in CHO-K1 Cells.