Electropolymerised molecularly imprinted polymers for the heat-transfer based detection of microorganisms: A proof-of-concept study using yeast
Electropolymerised molecularly imprinted polymers for the heat-transfer based detection of microorganisms: A proof-of-concept study using yeast
复制标题
DOI：
10.1016/j.tsep.2021.100956
复制
发表时间：
2021-05-07
影响因子：
4.8
通讯作者：
Peeters, M.
中科院分区：
文献类型：
--
作者：
Jamieson, O.;Betlem, K.;Peeters, M.
在这方面的贡献，分子印迹聚合物（MIPs）电聚合到丝网印刷碳电极（SPCE）开发特定的传感器用于热检测酵母。使用不含干扰物的实验室酵母菌株来优化聚合过程，而采用复杂混合物中的酵母（用于烘焙的酵母）来生产最终传感器并证明应用证明。采用两种不同的电聚合方法，分别为循环伏安法和计时电流法;电化学方法允许受控沉积和根据所需应用定制聚合物表面的能力。红外光谱和扫描电子显微镜证实，该方法导致不同的结构;与循环伏安法实现了高表面积，而计时电流法形成了致密的膜。随后，将这些功能化电极插入自制的热装置中，该热装置可以通过监测固液界面处的热阻（Rth）来测量酵母细胞与MIP层的选择性结合。测量结果显示，根据两种方法，MIP官能化电极产生MIP官能化电极的热信号的显著响应，而参比非印迹聚合物（NIP）官能化电极的情况并非如此。这表明，热分析可以用于检测酵母，即使是在复杂的样品，如食品。据我们所知，这是第一个报告的MIP电聚合到丝网印刷电极的真菌的热检测。所提出的方法能够使用与便携式设备兼容的简单制造过程快速生产低成本电极，这意味着高的商业潜力。在未来，这可以适用于包括细菌在内的各种微生物。
In this contribution, molecularly imprinted polymers (MIPs) were electropolymerised onto screen-printed carbon electrodes (SPCEs) to develop specific sensors for thermal detection of yeast. A laboratory yeast strain free of interferents was used to optimise the polymerisation procedure, whereas yeast in a complex mixture (yeast for baking) was employed to produce the final sensors and demonstrate proof-of-application. Two different electropolymerisation methods were employed, cyclic voltammetry and chronoamperometry respectively; the electrochemical methodology allows for controlled deposition and the ability to tailor the polymer surface to the required application. Infrared spectroscopy and scanning electron microscopy confirmed that the methods led to different structures; with cyclic voltammetry a high surface area was achieved, whereas for chronoamperometry a dense film was formed. Subsequently, these functionalised electrodes were inserted into a home-made thermal device that can measure the selective binding of yeast cells to the MIP layer via monitoring the thermal resistance (Rth) at the solid-liquid interface. The results of the measurements showed that MIP-functionalised electrodes produced, according to both methods, a significant response in thermal signal for the MIP-functionalised electrode, which was not the case for the reference Non-Imprinted Polymer (NIP)-functionalised electrode. This demonstrated that thermal analysis can be employed for the detection of yeast, even in a complex sample such as food. To our knowledge, this is the first report of MIPs electropolymerised onto screen-printed electrodes for the thermal detection of fungi. The proposed approach enables the fast production of low-cost electrodes using a simple manufacturing procedure compatible with a portable device, implying high commercial potential. In the future, this could be adapted to a broad range of microorganisms including bacteria.