Detection and cloning of new HTLV-related endogenous sequences in man.
Detection and cloning of new HTLV-related endogenous sequences in man.
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人类新 HTLV 相关内源序列的检测和克隆。
DOI：
10.1093/nar/17.17.6841
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发表时间：
1989
影响因子：
14.9
通讯作者：
Abraham,GN
中科院分区：
文献类型：
--
作者：
Perl,A;Rosenblatt,JD;Chen,IS;DiVincenzo,JP;Bever,R;Poiesz,BJ;Abraham,GN
从人类基因组文库中克隆了人类T-白血病病毒（HTLV）I型相关内源性序列（HRES）。HRES-1/1存在于所检查的所有正常供体的DNA中。通过核苷酸序列分析，HRES-1/1含有两个潜在的开放阅读框架，能够编码p25和p15。p25第一个ATG密码子5′端的684 bp侧翼区含有TATA盒、多聚腺苷酸化信号、推定的tRNA引物结合位点和逆转录病毒长末端重复序列的典型反向重复序列。系统发育分析表明，HRES-1/1可能作为外源性逆转录病毒进入灵长类动物的基因组。HRES-1/1 p25的氨基酸序列中，第6-36位与HTLV-Ⅰ和HTLV-Ⅱ的gag区同源性分别为32%和39%，第104-139位与人类免疫缺陷病毒2型（HIV-2）和猫肉瘤病毒（FSV）的gag区同源性分别为33%和28%。这表明感染灵长类动物的原始外源病毒可能在结构上是嵌合的。HRES-1/1基因组位点在淋巴细胞、黑素瘤细胞和胚胎组织中具有转录活性。
Human T-leukemia virus (HTLV) type I-related endogenous sequences (HRES) have been cloned from a human genomic libraty. HRES-1/1 is present in DNA of all normal donors examined. By nucleotide sequence analysis, HRES-1/1 contains two potential open reading frames capable of encoding a p25 and a p15. A 684 bp flanking region 5′ from the first ATG codon of p25 contains a TATA-box, a poly-adenylation signal, a putative tRNA primer binding site, and inverted repeats at locations which are typical of a retroviral long terminal repeat. Phylogenetic analysis suggests that HRES-1/1 entered the genome in primates, presumably as an exogenous retrovirus. From the deduced amino acid sequence of HRES-1/1 p25, residues 6–36 show a sequence homology of 32% and 39% to gag region segments of HTLV-I and HTLV-II, while residues 104–139 display a sequence homology of 33% and 28% to the gag regions of human immunodeficiency virus type 2 (HIV-2) and feline sarcoma vints (FSV), respectively. This suggests that the original exogenous virus infecting primate may be chimeric in structure. The HRES-1/1 genomic locus is transcriptionally active in lymphoid cells, melanoma cells, and embryonic tissues.