Monitoring and modelling of oxygen transport through un-crosslinked and crosslinked gelatine gels
Monitoring and modelling of oxygen transport through un-crosslinked and crosslinked gelatine gels
复制标题
DOI：
10.1016/j.polymertesting.2014.08.016
复制
发表时间：
2014-12-01
期刊：
影响因子：
5.1
通讯作者：
Tomlins, P.
中科院分区：
文献类型：
--
作者：
Elsayed, Y.;Lekakou, C.;Tomlins, P.
开发了一种无创的发光猝灭技术，用于在完全水合的明胶凝胶中连续监测氧气时空浓度分布，该凝胶旨在用作组织工程中的支架。使用了两个传质扩散模型来模拟系统中不稳定状态的氧气传输。确定水 - 凝胶界面处的氧扩散系数和传质系数，用于未连接的明胶，以及与1和1.5％和1.5％和1.5％w/v戊二醛的明胶交联。交联和交联剂的浓度增加降低了整个凝胶表面的氧气传递，但这两个因素都增加了大部分凝胶中氧的扩散系数。明胶微结构中的空隙是由于收缩和相关的内部应力而在交联过程中产生的，这两者都与凝胶内的扩散系数增加以及凝胶中氧气的不均匀扩散有关。 （c）2014 Elsevier Ltd.保留所有权利。
A non-invasive, luminescence quenching technique is developed for continuous monitoring of oxygen spatial-temporal concentration distribution in fully hydrated gelatine gels, intended for use as scaffolds in tissue engineering. Two mass transfer-diffusion models were used to simulate the unsteady-state oxygen mass transport in the system. Oxygen diffusion coefficient and mass transfer coefficient at the water-gel interface were determined for un-crosslinked gelatine, as well as gelatine crosslinked with 1 and 1.5% w/v glutaraldehyde. While crosslinking and increased concentration of the crosslinking agent reduced oxygen mass transfer across the gel surface, both factors increased the diffusion coefficient of oxygen in the bulk of the gel. Voids in the gelatine's microstructure, which were generated during the crosslinking process due to shrinkage and associated internal stresses, were associated with both increasing the diffusion coefficient within the gel, as well as inhomogeneous diffusion of oxygen within the gel. (C) 2014 Elsevier Ltd. All rights reserved.