Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells.
Immunologic relationship between platelet membrane glycoprotein GPIIb/IIIa and cell surface molecules expressed by a variety of cells.
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血小板膜糖蛋白GPIIb/IIIa与多种细胞表达的细胞表面分子之间的免疫关系。
DOI：
10.1073/pnas.83.16.6002
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发表时间：
1986
影响因子：
11.1
通讯作者：
Ginsberg,MH
中科院分区：
文献类型：
--
作者：
Plow,EF;Loftus,JC;Levin,EG;Fair,DS;Dixon,D;Forsyth,J;Ginsberg,MH
血小板膜糖蛋白GPIIb/IIIa的多克隆抗血清被用来检测抗原相关的分子在不同的面板上的人类细胞。脐静脉内皮细胞、红白血病HEL细胞和二倍体胎肺GM 1380成纤维细胞表达GPIIb/IIIa相关分子，通过表面标记蛋白的免疫荧光和免疫沉淀判断。GPIIb和GPIIIa亚基都存在，并且在这些细胞类型中具有相似的分子量。这些分子是细胞的合成产物，如内在标记蛋白质的免疫沉淀所示。4 β-佛波醇12-肉豆蔻酸酯13-乙酸酯可诱导早幼粒U937细胞合成并表达GPIIb/IIIa相关分子。GPIIb/IIIa相关分子在各种细胞类型中并不完全相同，这是基于电泳迁移率的微小差异以及它们未能与血小板GPIIb/IIIa每个亚基特异性的单克隆抗体反应。这些结果表明存在一个广泛分布的GPIIb/IIIa相关分子家族。这个家族的“细胞粘附素”可能在细胞粘附反应中具有共同的功能。
A polyclonal antiserum to platelet membrane glycoprotein GPIIb/IIIa was used to detect antigenically related molecules on a diverse panel of human cells. Umbilical vein endothelial cells, erythroleukemic HEL cells, and diploid fetal lung GM1380 fibroblasts expressed GPIIb/IIIa-related molecules, as judged by immunofluorescence and immunoprecipitation of surface-labeled proteins. The GPIIb and GPIIIa subunits were both present and were of similar molecular weight in these cell types. These molecules were synthetic products of the cells, as shown by immunoprecipitation of intrinsically labeled proteins. Promyeloid U937 cells could be induced by 4 beta-phorbol 12-myristate 13-acetate to synthesize and express GPIIb/IIIa-related molecules on their cell surface. The GPIIb/IIIa-related molecules were not precisely identical in the various cell types, based on slight differences in electrophoretic mobility and their failure to react with monoclonal antibodies specific for each subunit of platelet GPIIb/IIIa. These results suggest the existence of a widely distributed family of GPIIb/IIIa-related molecules. This family of "cytoadhesins" may share a common function in cellular adhesive reactions.