Maternal N-acetylcysteine suppresses fetal inflammatory cytokine responses to maternal lipopolysaccharide
Maternal N-acetylcysteine suppresses fetal inflammatory cytokine responses to maternal lipopolysaccharide
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DOI：
10.1016/j.ajog.2006.06.081
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发表时间：
2006-10-01
影响因子：
9.8
通讯作者：
Ross, Michael G.
中科院分区：
文献类型：
--
作者：
Beloosesky, Ron;Gayle, Dave A.;Ross, Michael G.
目的：有证据表明母体感染可诱导胎儿炎症反应。由于细胞因子的行动可能是由氧化应激介导的，我们确定是否N-乙酰半胱氨酸，抗氧化剂，可以钝胎儿炎症反应的母体lipopolysaccharide.Study design：Sprague道利近足月大鼠（n = 16）接受腹腔内脂多糖（100微克/公斤）在30分钟和盐水溶液或N-乙酰半胱氨酸（300毫克/公斤）在150分钟。另外一组在脂多糖给药前后接受N-乙酰半胱氨酸。在6小时，大鼠被杀死，胎儿和母亲的血液细胞因子determined.Results：母体脂多糖管理后，胎儿血液白细胞介素-6显着增加（3 +/-2至1265 +/-574 pg/mL）; N-乙酰半胱氨酸，给予之前或之前和之后脂多糖管理减少胎儿白细胞介素-6的反应控制水平。对于白细胞介素-1 β也观察到类似的趋势。N-乙酰半胱氨酸对胎儿白细胞介素-10水平没有影响observed.Conclusion：母体N-乙酰半胱氨酸抑制胎儿细胞因子对母体脂多糖的反应，即使在脂多糖注射后2小时。这些结果表明，N-乙酰半胱氨酸可以保护胎儿免受母体炎症后遗症的影响。(c)2006 Mosby，Inc. All rights reserved.
Objective: Evidence suggests that maternal infections may induce fetal inflammatory responses. Because cytokine actions may be mediated by oxidative stress, we determined whether N-acetylcysteine, an antioxidant, can blunt fetal inflammatory responses to maternal lipopolysaccharide.Study design: Sprague Dawley near-term rats (n = 16) received intraperitoneal lipopolysaccharide (100 mu g/kg) at 30 minutes and saline solution or N-acetylcysteine (300 mg/kg) at 150 minutes. An additional group received N-acetylcysteine before and after lipopolysaccharide administration. At 6 hours, rats were killed, and fetal and maternal blood cytokines were determined.Results: After maternal lipopolysaccharide administration, fetal blood interleukin-6 markedly increased (3 +/- 2 to 1265 +/- 574 pg/mL); N-acetylcysteine that was given before or before and after lipopolysaccharide administration reduced fetal interleukin-6 response to control levels. A similar trend was observed for interleukin-1 beta. No effect of N-acetyleysteine on fetal interleukin-10 levels was observed.Conclusion: Maternal N-acetyleysteine inhibits fetal cytokine responses to maternal lipopolysaccharide, even when given 2 hours after lipopolysaccharide injection. These results suggest that N-acetylcysteine may protect the fetus from sequelae of maternal inflammation. (c) 2006 Mosby, Inc. All rights reserved.