HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation.
HaCaT Keratinocytes Response on Antimicrobial Atelocollagen Substrates: Extent of Cytotoxicity, Cell Viability and Proliferation.
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DOI：
10.3390/jfb5020043
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发表时间：
2014-05-08
影响因子：
4.8
通讯作者：
Sáha P
中科院分区：
文献类型：
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作者：
López-García J;Lehocký M;Humpolíček P;Sáha P
经过广泛测试的有效杀菌剂：苯扎氯铵、溴硝醇、壳聚糖、氯己定和伊尔加桑以不同浓度添加到去端肽胶原基质中。为了评估这些抗菌剂如何影响角质形成细胞的生长，使用 MTT 测定测定细胞活力和增殖。获得的数据表明使用这些化学物质中的任何一种都具有低毒性。此外，细胞活力和增殖与没有杀菌剂的样品比较相似。这意味着无论使用哪种试剂，胶原蛋白细胞附着特性都不会因将这些杀菌剂掺入基材中而受到严重影响。因此，这些发现表明，通过添加一种或多种这些试剂增强的这些去端肽胶原底物可以提供对抗细菌污渍和生物膜形成的有效性，本文中称为“抗菌底物”的样品在可能适合组织工程应用的新型抗菌生物材料的设计中是一个有前景的观点。
The effective and widely tested biocides: Benzalkonium chloride, bronopol, chitosan, chlorhexidine and irgasan were added in different concentrations to atelocollagen matrices. In order to assess how these antibacterial agents influence keratinocytes cell growth, cell viability and proliferation were determined by using MTT assay. Acquired data indicated a low toxicity by employing any of these chemical substances. Furthermore, cell viability and proliferation were comparatively similar to the samples where there were no biocides. It means that regardless of the agent, collagen-cell-attachment properties are not drastically affected by the incorporation of those biocides into the substrate. Therefore, these findings suggest that these atelocollagen substrates enhanced by the addition of one or more of these agents may render effectiveness against bacterial stains and biofilm formation, being the samples referred to herein as “antimicrobial substrates” a promising view in the design of novel antimicrobial biomaterials potentially suitable for tissue engineering applications.