Prevalence and molecular characterization of Spirometra erinaceieuropaei spargana in snakes in Hunan Province, China
Prevalence and molecular characterization of Spirometra erinaceieuropaei spargana in snakes in Hunan Province, China
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DOI：
10.1017/s0022149x20000139
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发表时间：
2020-01-01
影响因子：
1.6
通讯作者：
Yang, L. C.
中科院分区：
文献类型：
--
作者：
Liu, W.;Tan, L.;Yang, L. C.
裂头蚴病是一种重要的食源性人畜共患寄生虫病；然而，关于中国湖南省蛇感染性尾蚴流行情况的报道很少。因此，我们对2018年该地区野生蛇的三棱蛇感染流行情况进行了调查，发现流行率高达91.7%（344/375）。 99.1%的乌梢蛇、94.1%的锦蛇和86.7%的绦蛇中有三棱寄生虫。寄生虫表现出不同的分布：50％位于肌肉组织，32.1％位于皮下组织，17.9％位于体腔。为了鉴定从野生蛇中收集的三棱柱的具体状态，对部分线粒体细胞色素c氧化酶亚基1（cox1）基因序列进行了扩增、测序和分析。所有检查的多尾蚴中 cox1 的序列变异范围在 0.0% 至 2.9% 之间，确定了 21 个变异位点 (4.71%, 21/446)。系统发育分析表明，湖南省分离的所有多尾蚴均为Spirometra erinaceieuropaei。这是湖南省首次报道蛇类感染猴头菌。应宣传裂头蚴病的风险和危害，控制非法野生动物贸易。
Sparganosis is an important foodborne parasitic zoonosis; however, few reports on the prevalence of snake-infecting plerocercoids from Hunan province in China are available. Therefore, we investigated the prevalence of spargana infection in wild snakes from this region in 2018, and identified an astonishing prevalence rate of 91.7% (344/375). Spargana parasites were found in 99.1% of Zaocys dhumnades, 94.1% of Elaphe carinata and 86.7% of Elaphe taeniura. Parasites exhibited various distributions: 50% were located in muscular tissue, 32.1% in subcutaneous tissue and 17.9% in the coelomic cavity. To identify the specific status of spargana collected from wild snakes, partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequences were amplified, sequenced and analysed. Sequence variations for cox1 among all the examined plerocercoids ranged between 0.0 and 2.9%, with 21 variable sites identified (4.71%, 21/446). Phylogenetic analyses identified that all plerocercoids isolated from Hunan province were Spirometra erinaceieuropaei. This is the first report of S. erinaceieuropaei infection in snakes in Hunan province. The risks and harms of sparganosis should be publicized, and illegal wildlife trade should be controlled.