Electroacupuncture induces c-Fos expression in the rostral ventrolateral medulla and peniaqueductal gray in cats: relation to opioid containing neurons
Electroacupuncture induces c-Fos expression in the rostral ventrolateral medulla and peniaqueductal gray in cats: relation to opioid containing neurons
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DOI：
10.1016/j.brainres.2004.09.059
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发表时间：
2004-12-24
期刊：
影响因子：
2.9
通讯作者：
Longhurst, JC
中科院分区：
文献类型：
--
作者：
Guo, ZL;Moazzami, AR;Longhurst, JC
我们以前的研究表明，电针内关-间石穴（P5-P6）可通过延髓头端腹外侧区（rVLM）的脑啡肽或β-内啡肽相关阿片受体抑制交感神经流出，减弱兴奋性内脏心血管反射。电针这些穴位是否激活rVLM和中脑导水管周围灰质（PAG）中含有脑啡肽或β-内啡肽的神经元尚不清楚，这些神经元参与电针介导的交感神经活动的中枢神经调节。本研究通过检测c-Fos免疫反应性来评估rVLM和PAG中激活的神经元，并确定了c-Fos核与这些区域中含有脑啡肽或β-内啡肽的神经元结构之间的关系。为了提高检测的细胞体含有脑啡肽或β-内啡肽，秋水仙碱（90-100 μ g/kg）注射到蛛网膜下腔麻醉猫28-30小时前EA或假手术对照EA。双侧去压力神经和颈迷走神经切断后，在P5-P6穴位（覆盖正中神经; n=7）进行EA（1-4 mA，2 Hz，0.5 ms）30 min。除了电刺激之外，在五只对照动物中进行。EA降低血压（BP）在4的7只猫（5-15毫米汞柱），而EA的假手术没有产生任何反应。在rVLM和PAG中发现含有脑啡肽的胞体，而在这两个区域中均未发现β-内啡肽标记的胞体。与对照组动物相比，更多的c-Fos免疫反应性。主要位于含有脑啡肽或β-内啡肽的纤维附近。在EA处理的猫的rVLM和腹外侧PAG（vlPAG）中观察到。电针后，猫rVLM内c-Fos和脑啡肽双标神经元明显增多（P
Our previous studies have shown that electroacupuncture (EA) at the Neiguan-Jianshi (P5-P6) acupoints inhibits sympathetic outflow and attenuates excitatory visceral cardiovascular reflexes through enkephalin or beta-endorphin-related opioid receptors in the rostral ventrolateral medulla (rVLM). It is not known whether EA at these acupoints activates neurons containing enkephalin or beta-endorphin in the rVLM as well as in the periaqueductal gray (PAG) that are involved in EA-mediated central neural regulation of sympathetic activity The present study evaluated activated neurons in the rVLM and PAG by detecting c-Fos immunoreactivity, and identified the relationship between c-Fos nuclei and neuronal structures containing enkephalin or beta-endorphin in these regions. To enhance the detection of cell bodies containing enkephalin or beta-endorphin, colchicine (90-100 mug/kg) was injected into the subarachnoid space in anesthetized cats 28-30 h prior to EA or the sham-operated control for EA. Following bilateral barodenervation and cervical vagotomy, EA (1-4 mA, 2 Hz, 0.5 ms) performed at the P5-P6 acupoints (overlying median nerve; n=7) for 30 min. Identical procedures. with the exception of electrical stimulation, were carried out in five control animals. EA decreased blood pressure (BP) in four of seven cats (5-15 mm Hg) while the sham procedure for EA produced no responses. Perikarya containing enkephalin were found in the rVLM and rarely in the PAG, while no cell bodies labeled with beta-endorphin were identified in either region. Compared to animals in the control group, more c-Fos immunoreactivity. located principally in close proximity to fibers containing enkephalin or beta-endorphin. was observed in the rVLM and ventrolateral PAG (vlPAG) in EA-treated cats. Moreover, neurons double-labeled with c-Fos and enkephalin in the rVLM were significantly increased in cats following EA stimulation (P