Facile preparation of carbohydrate microarrays by site-specific, covalent immobilization of unmodified carbohydrates on hydrazide-coated glass slides
Facile preparation of carbohydrate microarrays by site-specific, covalent immobilization of unmodified carbohydrates on hydrazide-coated glass slides
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DOI：
10.1021/ol051753z
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发表时间：
2005-09-15
期刊：
影响因子：
5.2
通讯作者：
Shin, I
中科院分区：
文献类型：
--
作者：
Lee, M;Shin, I
开发了一种新的、简单、高效的固定化方法，将单糖、双糖、寡糖和多糖固定在酰肼涂层的玻片上。蛋白质和细胞结合实验表明，该方法制备的碳水化合物微阵列适用于蛋白质-碳水化合物相互作用的快速分析和病原体的快速检测。
A new, simple and efficient immobilization method to attach mono-, di-, oligo-, and polysaccharides to hydrazide-coated glass slides was developed. Protein and cell-binding experiments show that the carbohydrate microarrays prepared by this method are applicable for the rapid analysis of protein-carbohydrate interactions and fast detection of pathogens.