Probiotic effects of orally administered Lactobacillus salivarius WB21-containing tablets on periodontopathic bacteria: a double-blinded, placebo-controlled, randomized clinical trial
Probiotic effects of orally administered Lactobacillus salivarius WB21-containing tablets on periodontopathic bacteria: a double-blinded, placebo-controlled, randomized clinical trial
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DOI：
10.1111/j.1600-051x.2009.01392.x
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发表时间：
2009-06-01
影响因子：
6.7
通讯作者：
Shimauchi, Hidetoshi
中科院分区：
文献类型：
--
作者：
Mayanagi, Gen;Kimura, Moto;Shimauchi, Hidetoshi
真柳G，木村M，中屋S，平田H，Sakamoto M，Benno Y，Shimuchi H。口服唾液乳杆菌WB21片剂对牙周病细菌的益生菌作用：一项双盲、安慰剂对照、随机临床试验。临床周期杂志2009；36：506-513。DOI：10.1111/j.1600-051X.2009.01392.x。本研究旨在评价口服乳酸菌是否可以改变牙周上/下菌斑中的细菌数量。66名无严重牙周炎的健康志愿者被随机分为两组，分别接受乳酸菌或安慰剂治疗8周(8w)：试验组(n=34)接受2.01×10(9)cfu/d的唾液乳杆菌WB21和木糖醇片剂；对照组(n=32)接受含木糖醇的安慰剂治疗。分别于治疗前、治疗4周(4周)和8周后(4周)和8周(8周)采集牙菌斑样本。采用实时定量聚合酶链式反应检测菌斑标本中的细菌数量，试验组中5种牙周病原菌在4W时菌斑中细菌总数显著减少[优势比(OR)=3.13，95%可信区间(CI)=1.28~7.65，P=0.012]。多因素分析显示，试验组在4周(OR=6.69，95%CI=2.51-17.9，p<；0.001)和8周(OR=3.67，95%CI=1.45-9.26，P=0.006)牙周菌斑中连翘单胞菌的减少几率均显著高于对照组。
Mayanagi G, Kimura M, Nakaya S, Hirata H, Sakamoto M, Benno Y, Shimauchi H. Probiotic effects of orally administered Lactobacillus salivarius WB21-containing tablets on periodontopathic bacteria: a double-blinded, placebo-controlled, randomized clinical trial. J Clin Periodontol 2009; 36: 506-513. doi: 10.1111/j.1600-051X.2009.01392.x.This study was designed to evaluate whether the oral administration of lactobacilli could change the bacterial population in supra/subgingival plaque.Sixty-six healthy volunteers without severe periodontitis were randomized into two groups to receive lactobacilli or placebo for 8 weeks (8W): the test group (n=34) received 2.01 x 10(9) CFU/day of Lactobacillus salivarius WB21 and xylitol in tablets; the control group (n=32) received placebo with xylitol. Supra/subgingival plaque samples were collected at the baseline and after 4 weeks (4W) and 8W. The bacterial amounts in plaque samples were analysed by quantitative real-time polymerase chain reaction.The numerical sum of five selected periodontopathic bacteria in the test group was decreased significantly in subgingival plaque at 4W [odds ratio (OR)=3.13, 95% confidence intervals (CI)=1.28-7.65, p=0.012]. Multivariate analysis showed that significantly higher odds were obtained for the reduction of Tannerella forsythia in subgingival plaque of the test group at both 4W (OR=6.69, 95% CI=2.51-17.9, p < 0.001) and 8W (OR=3.67, 95% CI=1.45-9.26, p=0.006).Oral administration of probiotic lactobacilli reduced the numerical sum of five selected periodontopathic bacteria and could contribute to the beneficial effects on periodontal conditions.