Extracellular high-mobility group 1 protein is essential for murine erythroleukaemia cell differentiation
Extracellular high-mobility group 1 protein is essential for murine erythroleukaemia cell differentiation
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DOI：
10.1042/bj3200253
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发表时间：
1996-11-15
影响因子：
4.1
通讯作者：
Pontremoli, S
中科院分区：
文献类型：
--
作者：
Sparatore, B;Passalacqua, M;Pontremoli, S
从鼠红白血病（MEL）细胞中分离的高迁移率族1（HMG 1）蛋白类型，当与诱导剂β-双乙酰胺一起加入MEL细胞培养物中时，可促进分化过程的加速。我们现在提供的直接证据表明，HMG 1蛋白在细胞外介质中的存在是必不可少的终末红细胞分化。HMG 1蛋白在MEL细胞中的细胞外功能进一步得到以下证明的支持：该蛋白从暴露于化学诱导剂的MEL细胞中释放，并且向细胞培养物中加入抗（HMG 1蛋白）单克隆抗体几乎完全抑制分化过程。HMG 1蛋白从MEL细胞的释放受影响细胞钙稳态的化合物如钙离子载体或维拉帕米调节。事实上，在离子载体的存在下，分化速率的增加伴随着HMG 1蛋白的细胞外释放的增强，而在维拉帕米的存在下，这两种现象都显著降低。
A high-mobility group 1 (HMG1) protein type isolated from murine erythroleukaemia (MEL) cells promotes acceleration of the differentiation process when added to a MEL cell culture together with the inducer hexamethylene bisacetamide. We now provide direct evidence that the presence of HMG1 protein in the extracellular medium is essential for terminal erythroid differentiation. An extracellular function for HMG1 protein in MEL cell is further supported by a demonstration that this protein is released from MEL cells exposed to the chemical inducer and that the addition of an anti-(HMG1 protein) monoclonal antibody to the cell culture inhibits the differentiation process almost completely. The release of HMG1 protein from MEL cells is modulated by compounds affecting cell calcium homoeostasis, such as a calcium ionophore or verapamil. In fact, in the presence of the ionophore an increased rate of differentiation is accompanied by an enhanced extracellular release of HMG1 protein, whereas in the presence of verapamil both phenomena are significantly decreased.