Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death
Neuroglobin and cytoglobin overexpression protects human SH-SY5Y neuroblastoma cells against oxidative stress-induced cell death
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DOI：
10.1016/j.neulet.2006.09.027
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发表时间：
2006-12-20
影响因子：
2.5
通讯作者：
Dewilde, Sylvia
中科院分区：
文献类型：
--
作者：
Fordel, Elke;Thijs, Liesbet;Dewilde, Sylvia
尽管生理浓度的ROS是细胞正常功能所必需的，但过量的ROS对细胞是有害的。脑红蛋白和细胞球蛋白是两种珠蛋白，其功能仍有争议。提示了其在ROS解毒中的潜在作用。探讨脑红蛋白和细胞球蛋白对人神经母细胞瘤SH-SY5Y细胞氧化应激后细胞死亡的影响。将SH-SY5Y细胞暴露于百草枯或过氧化氢可导致浓度和时间依赖的凋亡和坏死细胞死亡。与百草枯相比，过氧化氢诱导细胞死亡的效力是16倍。转染含有脑红蛋白或细胞珠蛋白序列的DNA的SH-SY5Y细胞在300 mU H_2O_2作用24 h后，其存活率明显高于未转染组。这一发现表明，脑红蛋白和细胞球蛋白保护SH-SY5Y细胞免受氧化应激诱导的细胞死亡。(C)2006年，爱思唯尔爱尔兰有限公司出版。
Although reactive oxygen species (ROS) at physiological concentrations are required for normal cell function, excessive production of ROS is detrimental to cells. Neuroglobin and cytoglobin are two globins, whose functions are still a matter of debate. A potential role in the detoxification of ROS is suggested. The influence of neuroglobin and cytoglobin on cell death after oxidative stress in human neuroblastoma SH-SY5Y cells was evaluated. Exposure of SH-SY5Y cells to paraquat or H2O2 resulted in a concentration- and time-dependent induction of apoptotic and necrotic cell death. H2O2 was 16 times more potent to induce cell death as compared to paraquat. SH-SY5Y cells transfected with plasmid DNA containing the neuroglobin or cytoglobin sequence showed enhanced survival after exposure to 300 mu M H2O2 for 24 h as compared to untransfected controls. This finding suggests that neuroglobin and cytoglobin protect SH-SY5Y cells against oxidative stress-induced cell death. (c) 2006 Published by Elsevier Ireland Ltd.