GERM CELL-SPECIFIC EXPRESSION OF A GENE ENCODING EUKARYOTIC TRANSLATION ELONGATION FACTOR-1-ALPHA (EEF-1-ALPHA) AND GENERATION OF EEF-1-ALPHA RETROPSEUDOGENES IN XENOPUS-LAEVIS
GERM CELL-SPECIFIC EXPRESSION OF A GENE ENCODING EUKARYOTIC TRANSLATION ELONGATION FACTOR-1-ALPHA (EEF-1-ALPHA) AND GENERATION OF EEF-1-ALPHA RETROPSEUDOGENES IN XENOPUS-LAEVIS
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DOI：
10.1073/pnas.88.20.9277
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
MAZABRAUD, A
中科院分区：
文献类型：
--
作者：
ABDALLAH, B;HOURDRY, J;MAZABRAUD, A
我们已经研究了通过原位杂交的基因编码的体细胞形式和卵母细胞形式的非洲爪蟾eEF-1-α的表达。体细胞形式的eEF-1-alpha（eEF-1-alpha-S）mRNA在成年性腺的雄性和雌性生殖细胞中几乎检测不到，但在神经胚阶段后的胚胎细胞中非常丰富。相比之下，另一种形式的eEF-1-alpha（eEF-1-alpha-O）mRNA在卵原细胞和卵黄发生前卵母细胞中高度浓缩，但在卵子和胚胎中检测不到。eEF-1-alpha-O mRNA也存在于成年睾丸的精原细胞和精母细胞中。后一项发现将eEF-1-alpha-O mRNA鉴定为生殖细胞特异性基因产物。虽然生殖细胞中含有很少的eEF-1-alpha-S mRNA，但在X.光滑染色体这些基因被认为在生殖细胞中由mRNA的逆转录和随后的cDNA拷贝整合到染色体DNA中产生。我们认为eEF-1-α-S假基因是在胚胎的原始生殖细胞分化为卵原细胞或精原细胞之前产生的。
We have studied by in situ hybridization the expression of the genes encoding the somatic form and the oocyte form of Xenopus laevis eEF-1-alpha. The somatic form of eEF-1-alpha (eEF-1-alpha-S) mRNA is virtually undetectable in male and female germ cells of the adult gonad but is very abundant in embryonic cells after the neurula stage. In contrast, another form of eEF-1-alpha (eEF-1-alpha-O) mRNA is highly concentrated in oogonia and in previtellogenic oocytes but is undetectable in eggs and embryos. eEF-1-alpha-O mRNA is also present in spermatogonia and spermatocytes of adult testis. The latter finding identifies eEF-1-alpha-O mRNA as a germ cell-specific gene product. Although germ cells contain very little eEF-1-alpha-S mRNA, several eEF-1-alpha-S retropseudogenes exist in X. laevis chromosomes. These genes are thought to arise in germ cells from reverse transcription of mRNA and subsequent integration of the cDNA copies into chromosomal DNA. We suggest that eEF-1-alpha-S pseudogenes are generated in primordial germ cells of the embryo before they differentiate into oogonia or spermatogonia.