Sequence and expression analysis of two T helper master transcription factors, T-bet and GATA3, in rainbow trout Oncorhynchus mykiss and analysis of their expression during bacterial and parasitic infection
Sequence and expression analysis of two T helper master transcription factors, T-bet and GATA3, in rainbow trout Oncorhynchus mykiss and analysis of their expression during bacterial and parasitic infection
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DOI：
10.1016/j.fsi.2010.06.016
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发表时间：
2010-11-01
影响因子：
4.7
通讯作者：
Secombes, Christopher J.
中科院分区：
文献类型：
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作者：
Wang, Tiehui;Holland, Jason W.;Secombes, Christopher J.
初始CD 4 + T细胞向辅助性T细胞（Th）1或Th 2细胞的极化由两个主转录因子T-bet和GATA 3指定，并且是哺乳动物对病原体的适应性免疫应答和持久免疫的发展的基本特征。我们在这里报告虹鳟鱼Mykiss T-bet和GATA 3的克隆，让未来的评估鲑鱼Th 1和Th 2细胞的存在。鳟鱼T-bet翻译与其他鱼类T-bet分子具有较高的氨基酸同源性（71-72%），但与哺乳动物T-bet基因的同源性较低（41-42%），尽管中间T-box DNA结合结构域在所有来自鱼类和哺乳动物的T-bet蛋白中高度保守。鳟鱼GATA 3与所有已知脊椎动物分子具有高氨基酸序列同一性（73-88%），具有两个高度保守的锌指基序。系统发育树分析结果表明，T-bet和GATA 3在健康鳟鱼的脾脏、头肾和肌肉中表达量相近，而GATA 3在鳃、脑、皮肤和肠中的表达量高于T-bet。T-bet和GATA 3的表达受不同刺激物的调节。T细胞刺激剂PHA可上调脾细胞中T-bet和GATA 3的表达，提示它们可能主要由活化的T细胞表达。T-bet和GATA 3在脾脏中的表达在急性应激中增加，但它们的表达被细菌（鲁氏耶尔森氏菌）感染抑制。在寄生虫感染模型中，苔藓四囊藻感染诱导了偏向性基因表达谱，其中观察到T-bet、IFN-gamma和IL-2的表达大幅增加，这表明这种疾病可能诱导了Th 1样反应。更好地了解病原体调节的T-bet和GATA 3的表达，以及潜在的潜在的宿主免疫反应引起的结果，其表达，可能会允许新的未来控制措施，对鱼类疾病的发展。(C)2010爱思唯尔有限公司保留所有权利。
The polarization of naive CD4+ T cells to T helper (Th)1 or Th2 cells is specified by two master transcription factors, T-bet and GATA3, and is an essential feature of mammalian adaptive immune responses to pathogens and the development of long-lasting immunity. We report here the cloning of rainbow trout Oncorhynchus mykiss T-bet and GATA3, to allow the future evaluation of the existence of Th1 and Th2 cells in salmonid fish. The trout T-bet translation shares high amino acid identities to other fish T-bet molecules (71-72%) but low identities to mammalian T-bet genes (41-42%), although the middle T-box DNA binding domain is highly conserved among all the T-bet proteins from fish and mammals. The trout GATA3 has high amino acid sequence identities (73-88%) to all known vertebrate molecules, with two highly conserved zinc finger motifs. The identity of the trout T-bet and GATA3 molecules was confirmed by phylogenetic tree analysis.A comparable expression level of T-bet and GATA3 was seen in the spleen, head kidney and muscle in healthy trout, but a higher expression level of GATA3 was seen in the gills, brain, skin and intestine relative to that of T-bet. T-bet and GATA3 expression was modulated by different stimulants. The T cell stimulant PHA up-regulated the expression of both T-bet and GATA3 in splenocytes, suggesting that they may be mainly expressed by activated T cells. The expression of T-bet and GATA3 in the spleen was increased by acute stress, but their expression was inhibited by bacterial (Yersinia ruckeri) infection. In a parasitic infection model, Tetracapsuloides bryosalmonae infection induced a biased gene expression profile where a large increase in the expression of T-bet, IFN-gamma and IL-2 was seen, suggesting that a Th1-like response is likely induced by this disease. A better understanding of pathogen modulated expression of T-bet and GATA3, and the potential underlying host immune responses elicited as a consequence of their expression, may allow novel future control measures against disease in fish to be developed. (C) 2010 Elsevier Ltd. All rights reserved.