Polarization of human hematopoietic progenitors during contact with multipotent mesenchymal stromal cells:: Effects on proliferation and clonogenicity
Polarization of human hematopoietic progenitors during contact with multipotent mesenchymal stromal cells:: Effects on proliferation and clonogenicity
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DOI：
10.1089/scd.2006.15.815
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发表时间：
2006-12-01
影响因子：
4
通讯作者：
Corbeil, Denis
中科院分区：
文献类型：
--
作者：
Freund, Daniel;Bauer, Nicola;Corbeil, Denis
建立明确的细胞培养系统，促进分离的造血干细胞和祖细胞（HSPC）的离体扩增，是血液学和干细胞移植中的一个关键问题。在这里，我们评估了原代人多能间充质基质细胞（MSC）支持外周富含CD34（+）的HSPC体外扩增的能力。我们观察到，与未在 MSC 上共培养的 HSPC 相比，在 MSC 上共培养的 HSPC 表现出明显更高的总扩增率。此外，除了CD34(+)CD133(+)和CD34(+)CD133(+)细胞的扩增之外，第三群CD133(+)CD34(+)干细胞在扩增后变得可检测到。与 CD34(+)CD133(+) 细胞相比，HSPC 与饲养层之间的直接接触似乎有利于具有 CD133(+) 表型（即 CD34(+)CD133(+) 和 CD133(+)CD34(+)）的 HSPC 的扩增。有趣的是，电子显微镜和免疫荧光分析表明贴壁的 HSPC 表现出不同的形态。它们要么是圆形的，在某些情况下具有微绒柱的外观，要么表现出几种不同类型的质膜突起，例如片状足和马状足。 CD133选择性地集中在其中，而CD34随机分布在HSPC的整个表面上。总之，这种共培养提供了一个独特的实验系统，可以进一步表征稀有干细胞群标记的生物学和作用。
Establishment of a defined cell culture system that facilitates ex vivo expansion of isolated hematopoietic stem and progenitor cells (HSPCs) is a crucial issue in hematology and stem cell transplantation. Here we have evaluated the capacity of primary human multipotent mesenchymal stromal cells (MSCs) to support the ex vivo expansion of peripheral CD34(+)-enriched HSPCs. We observed that HSPCs co-cultured on MSCs showed a substantially higher total expansion rate compared to those growing without. Moreover, in addition to the expansion of CD34(+)CD133(+) and CD34(+)CD133(+) cells, a third population of CD133(+)CD34(+) stem cells became detectable after expansion. Direct contact between HSPCs and the feeder layer appears beneficial for the expansion of HSPCs harboring CD133(+) phenotype, i.e., CD34(+)CD133(+) and CD133(+)CD34(+), in contrast to CD34(+)CD133(+) cells. Interestingly, electron microscopy and immunofluorescence analyses revealed that adherent HSPCs display various morphologies; they are either round with, in some cases, the appearance of a microvillar pole or exhibit several distinct types of plasma membrane protrusions such as lamellipodium and magnupodium. CD133 is selectively concentrated therein, whereas CD34 is randomly distributed over the entire surface of HSPCs. Together, this co-culture offers a unique experimental system to further characterize the biology and role of markers of rare stem cell populations.