STUDY OF THE DEVELOPMENT OF THE HEMATOPOIETIC SYSTEM USING QUAIL-CHICK CHIMERAS OBTAINED BY BLASTODERM RECOMBINATION
STUDY OF THE DEVELOPMENT OF THE HEMATOPOIETIC SYSTEM USING QUAIL-CHICK CHIMERAS OBTAINED BY BLASTODERM RECOMBINATION
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DOI：
10.1016/0012-1606(80)90165-7
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
DIETERLENLIEVRE, F
中科院分区：
文献类型：
--
作者：
MARTIN, C;BEAUPAIN, D;DIETERLENLIEVRE, F
用鹌鹑胚盘透明区前部延伸至第10或第17体节水平的部分代替鸡卵内胚盘的相应区域。进行了相互移植。在外观上，所得嵌合体具有复合体，1种。头和颈或头、颈和翅区的部分由其它物种提供，其余部分由其它物种提供。嵌合体总是被移植到鸡的卵黄囊上。用鹌鹑-鸡细胞核差异法分析了39只存活嵌合体在孵育11-13天后造血器官的细胞组成。胸腺的组成取决于2种属之间的边界水平。在嵌合体中，鹌鹑贡献的头部和颈部，胸腺上皮基质的鹌鹑细胞，而淋巴细胞群的鸡的起源。当鹌鹑的贡献也延伸到翅膀，胸腺基质和淋巴细胞的鹌鹑起源。在相互组合，其中头部和颈部是鸡源鹌鹑身体，得到了不同的结果：没有淋巴细胞存在于胸腺中，这是减少到其上皮成分，这是鸡源。如果鸡的贡献延伸到翅膀，在互惠组合中，所有胸腺成分都是鸡源性的。脾脏和腔上囊在大多数情况下并没有不同的细胞组成从周围的组织，但在一些嵌合体的细胞的其他species. Hemopoietic注定要殖民胚胎内器官的细胞混合物出现在领土来自整个透明区，不包括前瞻性的头颈部地区。每一个造血器官的雏形似乎是由来自邻近地区的前体殖民。
The anterior part of the area pellucida from quail blastoderms extending to the 10th or the 17th somite level was substituted for the corresponding region of chick blastoderms in ovo. Reciprocal grafts were carried out. In external appearance the resulting chimeras had a composite body, 1 spp. contributing the head and neck or the head, neck and wing regions while the other species provided the remainder. The chimeras were always grafted on a chick yolk sac. The cellular composition of hemopoietic organs according to species was analyzed by the quail-chick nuclear difference in 39 viable chimeras at 11-13 days of incubation. The thymus composition depended on the level of the boundary between the 2 spp. In chimeras in which the quail contributed head and neck, the thymic epithelial stroma was made of quail cells while the lymphoid population was of chick origin. When the quail contribution also extended to the wings, thymic stroma and lymphoid cells were of quail origin. In reciprocal combinations, in which head and neck were of chicken origin on a quail body, a different result was obtained: no lymphoid cells were present in the thymus which was reduced to its epithelial component and this was of chick origin. If the chick contribution extended to the wings, as in the reciprocal combination, all thymus components were of chick origin. The spleen and the bursa of Fabricius in most instances did not differ in their cellular composition from the surrounding tissues; however in some chimeras a minor admixture of cells of the other species was found. Hemopoietic stem cells destined to colonize intraembryonic organs arise in territories derived from the whole area pellucida excluding the prospective head-neck region. Each hemopoietic organ rudiment appears to be colonized by precursors derived from adjacent territories.