Efficacy of an orange oil emulsion as an anthelmintic against Haemonchus contortus in gerbils (Meriones unguiculatus) and in sheep
Efficacy of an orange oil emulsion as an anthelmintic against Haemonchus contortus in gerbils (Meriones unguiculatus) and in sheep
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DOI：
10.1016/j.vetpar.2010.04.017
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发表时间：
2010-08-27
影响因子：
2.6
通讯作者：
Zajac, Anne M.
中科院分区：
文献类型：
--
作者：
Squires, Jill M.;Foster, Joyce G.;Zajac, Anne M.
弯曲血蜱是一种对世界范围内的小型反刍动物生产者造成重大损失的吸血性胃粘膜寄生虫，近年来抗驱虫寄生虫数量的增加导致了对替代控制方法的需求。一种橙油乳剂在体外对植物寄生线虫和弯曲血蜱有活性，并在沙鼠模型和天然羊宿主中进行了对弯曲血蜱的活性评估600感染性第三阶段(13)H contortus幼虫在一次实验中沙鼠服用600毫克每千克体重(毫克/公斤)橙油连续5天每天一次或在第二个实验中沙鼠服用1200毫克/公斤橙油一次或每天5天9天沙鼠感染后被杀害他们的胃切除和蠕虫造成的600毫克/公斤体重剂量减少7%和62年6%的寄生虫与对照组相比在一次或每天5天1200毫克/公斤体重剂量分别减少25%和87年8%的寄生虫引起的橙油相比,对照组分别在连续5天每天一次或多次治疗,对照组之间的差异在两个剂量显著(P < 0 005)在18羊羔羊试验口服接种10 000 13 H contortus一个月后两组羊羔六只每个服用600毫克/公斤橙油一次或每天3天粪蛋数量从治疗的第一天（第0天）开始，持续监测14天，结果表明，与对照组相比，单剂量产品导致粪蛋数量大幅减少（94%），到第2天，鸡蛋数量显著减少（P< 0 0001），因此橙油乳剂可能对控制绵羊血液病有用(C) 2010
Haemonchus contortus is a blood-sucking abomasal parasite responsible for major losses to small ruminant producers worldwide The recent increase in populations of anthelmintic resistant parasites has produced a demand for alternative control methods An orange oil emulsion that has shown activity against plant parasitic nematodes and H contortus in vitro was assessed for activity against H contortus in a gerbil model and in the natural ovine host In gerbil experiments animals were infected with 600 infective third stage (13) H contortus larvae In one experiment gerbils were treated with 600 milligrams per kilogram bodyweight (mg/kg BW) orange oil once or daily for 5 days In a second experiment gerbils were treated with 1200 mg/kg BW orange oil once or daily for 5 days On Day 9 post-infection gerbils were killed their stomachs removed and the worms counted The 600 mg/kg BW dosage caused 7% and 62 6% parasite reduction compared to a control group when given once or daily for 5 days respectively The 1200 mg/kg BW dosage of orange oil caused 25% and 87 8% parasite reduction compared to a control group when given once or daily for 5 days respectively The difference between the multiple treatment and control group were significant at both dosages (P< 0 005) In the sheep trial 18 lambs were orally inoculated with 10 000 13 H contortus One month later two groups of six lambs each were dosed with 600 mg/kg BW orange oil either once or daily for 3 days Fecal egg counts were monitored daily starting on the first day of treatment (Day 0) and continuing for 14 days Results showed that a single dose of the product caused high fecal egg count reduction (97 4%) compared to control sheep Egg counts were significantly reduced by Day 2 (P< 0 0001) Thus the orange oil emulsion may potentially be useful in the control of ovine haemonchosis (C) 2010 Elsevier B V All rights reserved