Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats.
Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats.
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DOI：
10.1155/2013/284025
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Wang N
中科院分区：
文献类型：
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作者：
Tan S;Wang G;Guo Y;Gui D;Wang N
本研究观察黄芪甲苷(AS-IV)对大鼠肾缺血再灌注(IR)所致急性肾损伤(AKI)的影响。采用双侧肾动脉夹闭45 ，再灌流12 h和24 h的方法复制大鼠急性肾损伤模型。AS-IV以10和20 ·kg~(-1)−·d~(-1)−-1灌胃，1次/d，连续7d。AS-IV可显著降低急性脑损伤大鼠再灌流12 h和24 h的血清尿素、肌酐和胱抑素C水平。AS-IV可减轻急性肾损伤大鼠肾小管损伤，抑制NF-κB p65亚单位的磷酸化。血清和组织中κ-α、单核细胞趋化蛋白-1和细胞间黏附分子-1水平升高。所有这些异常都被AS-IV预防。此外，AS-IV还下调急性脑损伤大鼠肺组织中NF-κB、α、单核细胞趋化蛋白1和细胞间黏附分子-1的表达。这些结果提示AS-IV可能通过抑制NF-κB介导炎性基因的表达而成为预防缺血性急性脑损伤的一种新的治疗方法。
This study investigated the anti-inflammatory effects of astragaloside IV(AS-IV) on ischemia/reperfusion (IR) induced acute kidney injury (AKI) in rats. Experimental model of ischemic AKI was induced in rats by bilateral renal artery clamp for 45 min followed by reperfusion of 12 h and 24 h, respectively. AS-IV was orally administered once a day to rats at 10 and 20 mg·kg−1·d−1 for 7 days prior to ischemia. AS-IV pretreatment significantly decreased serum urea, creatinine, and cystatin C levels at 12 h and 24 h of reperfusion in AKI rats. AS-IV pretreatment also ameliorated tubular damage and suppressed the phosphorylation of p65 subunit of NF-κB in AKI rats. Moreover, NF-κB and MPO activity as well as serum and tissue levels of TNF-α, MCP-1, and ICAM-1 were elevated in AKI rats. All of these abnormalities were prevented by AS-IV. Furthermore, AS-IV downregulated the mRNA expression of NF-κB, TNF-α, MCP-1, and ICAM-1 in AKI rats. These results suggest that AS-IV might be developed as a novel therapeutic approach to prevent ischemic AKI through inhibition of NF-κB mediated inflammatory genes expression.