Glycolipids: Receptors for fibronectin?
Glycolipids: Receptors for fibronectin?
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DOI：
10.1002/jcp.1041090218
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发表时间：
1981-11
影响因子：
5.6
通讯作者：
K. Yamada;D. Kennedy;Gary R. Grotendorst;T. Momoi
中科院分区：
文献类型：
--
作者：
K. Yamada;D. Kennedy;Gary R. Grotendorst;T. Momoi
我们用三种不同的生物测定系统检验了糖脂可能作为细胞表面糖蛋白纤维连接蛋白受体的假设。我们发现纯化的可溶性神经节苷抑制纤维连接蛋白介导的血凝、细胞扩散和转化细胞正常形态表型的恢复。抑制作用是剂量依赖性和竞争性的；2 μg/ml纤维连接蛋白的血凝作用被小于1 μM的神经节苷抑制一半。最有效的神经节苷类抑制剂通常含有最多的唾液酸残基。神经节苷脂的分离寡糖部分保留了这种抑制活性，含有更多唾液酸的寡糖是更有效的抑制剂。
We have examined the hypothesis that glycolipids might serve as receptors for the cell surface glycoprotein fibronectin using three different biological assay systems. We find that purified solubilized gangliosides inhibit fibronectin‐mediated hemagglutination, cell spreading, and restoration of a normal morphologic phenotype to transformed cells. The inhibition is dose‐dependent and competitive; hemagglutination by 2 μg/ml fibronectin is half‐maximally inhibited by less than 1 μM gangliosides. The most effective ganglioside inhibitors generally contain the most sialic acid residues. The isolated oligosaccharide portions of gangliosides retain this inhibitory activity and the oligosaccharides with more sialic acid are more effective inhibitors.