Evaluation of freezing tolerance inActinidiagermplasm based on relative electrolyte leakage
Evaluation of freezing tolerance inActinidiagermplasm based on relative electrolyte leakage
复制标题
基于相对电解质渗漏的猕猴桃质体耐冻性评价
DOI：
10.1007/s13580-020-00272-4
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发表时间：
2020
影响因子：
2.4
通讯作者：
Fang Jinbao
中科院分区：
文献类型：
--
作者：
Sun Shihang;Qi Xiujuan;Wang Ran;Lin Miaomiao;Fang Jinbao
冬季低温胁迫会对猕猴桃的产量产生灾难性的影响，并影响其地理分布。然而，冷冻耐受性在猕猴桃成岩型仍然在很大程度上未知。在这里，我们报告了暴露于低温胁迫（− 5 °C，− 10 °C，− 15 ° C，− 20 °C，− 25 °C和− 30 °C）的Actinidiagenotypes芽中代谢物含量和酶活性的变化。此外，还采用相对电解质渗漏法对猕猴桃种质资源的抗冻性进行了评价，共对猕猴桃属16个种的51个基因型进行了抗寒性评价。结果表明，相对电解质渗漏率、脯氨酸（Pro）、可溶性蛋白和过氧化氢酶（CAT）活性随低温的变化而变化。结果表明，在16种昆虫中，A. kolomikta，A. polygama和A.结果表明，紫茎泽兰的LT_（50）低于其它物种。原产于东北地区的紫茎泽兰具有较强的抗冻性。A. chinensisandA.美味。这些发现为猕猴桃的抗冻能力和机制提供了新的见解，并进一步有助于我们理解抗冻性与地理分布之间的关系。
Cold stress in winter can have a disastrous effect on kiwifruit yield and affect geographical distribution. However, freezing tolerance inActinidiagenotypes remains largely unknown. Here, we report changes in metabolite content and enzyme activity in the shoots ofActinidiagenotypes exposed to low-temperature stress (− 5 °C, − 10 °C, − 15 °C, − 20 °C, − 25 °C and − 30 °C). Moreover, the relative electrolyte leakage method was used to evaluate the freezing tolerance of kiwifruit germplasm; 51 genotypes from 16 species ofActinidiawere evaluated in total. The data revealed that relative electrolyte leakage, proline (Pro), soluble protein, and catalase (CAT) activity changed with different low temperatures. Results showed that among 16 species,A. kolomikta,A. polygama, andA. argutahad lower LT50 than other species.A. arguta, originating from the northeast of China, exhibited stronger freezing resistance than the ones from other places. There was little difference in freezing tolerance betweenA. chinensisandA. deliciosa. These findings provide new insights into the freezing tolerance ability and mechanisms of kiwifruit and further contribute to our understanding of the relationship between freezing tolerance and geographic distribution.