Determination of Glucose by a Modification of Somogyi-Nelson Method
Determination of Glucose by a Modification of Somogyi-Nelson Method
复制标题
改进的 Somogyi-Nelson 法测定葡萄糖
DOI：
10.1271/bbb1961.44.2943
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Y. Kobara
中科院分区：
文献类型：
--
作者：
C. Hatanaka;Y. Kobara
Nelson’s arsenomolybdate, the chromogenic reagent in Somogyi–Nelson method, was replaced by Folin–Ciocalteu phenol reagent. The major object was to remove the toxic arsenic compounds from the color reaction system. The color-producing ability of the phenol reagent was considerably lower than that of Nelson’s reagent. However, the modified method was favorably comparable to Somogyi–Nelson method in simplicity, reproducibility and stability of color development. The error in both the modified and Somogyi–Nelson method could be reduced to about one fourth by adding sodium benzoate (final concentration, about 0.5%) to the test solutions.
