Methyl jasmonate stimulates polyamines biosynthesis and resistance against leaf rust in wheat plants
Methyl jasmonate stimulates polyamines biosynthesis and resistance against leaf rust in wheat plants
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DOI：
10.1080/03235400600914355
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发表时间：
2009-01-01
影响因子：
1
通讯作者：
Abd-El-Kareem, F.
中科院分区：
文献类型：
--
作者：
Haggag, Wafaa M.;Abd-El-Kareem, F.
小麦（Triticum aestivum L.）叶锈病，叶锈菌Puccinia recondita ex Desm。F.埃及小麦主要病害之一。茉莉酸甲酯（MJ）是一种潜在的植物诱导子，能诱导针叶树产生多种化学和解剖防御反应，可用于提高针叶树对生物伤害的系统抗性。在温室中，应用不同浓度的MJ（10、20和30 mM）作为种子浸泡加叶面喷雾或仅作为叶面喷雾来控制叶锈病和诱导小麦植物叶片中的次生化合物产生。在播种30天和50天后施用叶面喷雾。结果表明，不同浓度和处理均能降低小麦锈病的发生程度。在45天的接种过程中，小麦叶片中的小麦黑胫病菌。当与10 mM MJ或对照植物相比时，在MJ处理的植物中，作为种子浸泡加用20和30 mM叶面喷雾，疾病发病率显著降低。研究表明，随着MJ浓度的增加，次生代谢产物大大增加。内源性水平的自由和共轭腐胺，亚精胺和精胺增加响应于激发子。多胺生物合成酶的鸟氨酸脱羧酶（ODC）和多胺氧化酶（PAO）的活性显示高达三倍的增加相对于未处理的对照。此外，与未处理和受感染的植物相比，处理植物中的植物防御相关蛋白、过氧化物酶和几丁质酶的活性以及游离酚和结合酚的含量显著增加。此外，MJ处理还提高了叶绿素a、叶绿素b和类胡萝卜素含量，其中以20和30 mM MJ浸种+叶面喷施处理的提高幅度最大。在田间条件下，采用10、20和30 mM MJ浸种+叶面喷施或单独叶面喷施3种浓度的MJ，研究其对小麦锈病的防治效果。在播种30天和80天后施用叶面喷雾。结果表明，与其他处理和对照相比，浸种+叶面喷施20和30 mM MJ的组合处理可显著降低病害严重程度。同时，各处理均提高了小麦植株的生长和籽粒产量。因此，茉莉酸甲酯浸种与叶面喷施相结合的组合处理在田间防治小麦锈病上具有一定的应用价值。
Leaf rust of wheat (Triticum aestivum L.), incited by Puccinia recondita ex Desm. f. sp. tritici Eriks, is one of the most important wheat diseases in Egypt. Methyl jasmonate (MJ) is a potential plant elicitor which induces a wide range of chemical and anatomical defense reactions in conifers and might be used to increase systemic resistance against biotic damage. In the greenhouse, different concentrations of MJ (10, 20 and 30 mM) were applied as seed soaking plus foliar spray or only as foliar spray to control leaf rust and induction of secondary compound production in leaves of wheat plants. Foliar spray was applied after 30 and 50 days of sowing. Results indicated that all concentrations and treatments reduced the severity of rust disease caused by P. recondita f. sp. tritici in wheat leaves during 45 days of inoculations. Disease incidence was decreased significantly in MJ-treated plants as seed soaking plus foliar spray with 20 and 30 mM when compared to 10 mM MJ or control plants. The study revealed that, with increasing concentrations of MJ, the secondary metabolites were greatly increased. Endogenous levels of both free and conjugated putrescine, spermidine and spermine increased in response to the elicitor. Activities of polyamine biosynthetic enzymes of ornithine decarboxylase (ODC) and polyamine oxidase (PAO) displayed up to threefold increases relative to untreated control. Moreover, significant increases in activities of plant defense-related protein, enzymes as peroxidase and chitinase as well as free and conjugated phenols contents were recorded in treated plants compared with untreated and infected plants. Furthermore, MJ treatment increased the chlorophyll-a, chlorophyll-b and carotenoids pigments contents, the higher increase was obtained with combined treatment between seeds soaking plus foliar spray at 20 and 30 mM of MJ. Under field conditions, three concentrations of MJ, i.e. 10, 20 and 30 mM as combined treatment between seeds soaking plus foliar spray or only as foliar spray were applied to study their effect against rust disease. Foliar spray was applied after 30 and 80 days of sowing. Results showed that the high reduction in disease severity was obtained with combined treatments between seeds soaking plus foliar spray with MJ at 20 and 30 mM compared with other treatments and control. At the same time, all treatments increased the growth and grain yield of wheat plants. It could be suggested that combination treatment between seeds soaking plus foliar spray with methyl jasmonate might be used commercially for controlling rust disease of wheat plants under field conditions.