Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke.
Limb remote ischemic per-conditioning in combination with post-conditioning reduces brain damage and promotes neuroglobin expression in the rat brain after ischemic stroke.
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DOI：
10.3233/rnn-140413
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发表时间：
2015
影响因子：
2.8
通讯作者：
Ji X
中科院分区：
文献类型：
--
作者：
Ren C;Wang P;Wang B;Li N;Li W;Zhang C;Jin K;Ji X
目的：肢体远端缺血预处理或后处理已被证明对脑缺血性卒中具有神经保护作用。然而，远程预处理与后处理相结合对缺血/再灌注损伤的影响以及潜在的机制在很大程度上是未知的。 方法：成年雄性Sprague道利大鼠，大脑中动脉闭塞（MCAO）。在局灶性缺血发作后立即施加肢体缺血刺激（预处理），然后在再灌注后24小时重复短暂的远程缺血（后处理）。观察再灌注后不同时间脑梗死体积、运动功能及脑红蛋白（Ngb）表达的变化。 结果如下：我们发现，单次肢体远程预处理提供了短期的保护，但在再灌注后的14天内，结合重复的远程后处理显着改善脑缺血/再灌注损伤。有趣的是，我们还发现，缺血预处理和后处理显著增加了Ngb的表达，Ngb是一种氧结合球蛋白，已被证明对缺血/再灌注后第1天至第14天的梗死周围区域具有神经保护作用。结论：我们的研究结果表明，传统的预处理结合后处理可能是一种新的神经保护策略，对缺血再灌注损伤，Ngb似乎是一个重要的球员在肢体远程缺血介导的神经保护。
Purpose: Limb remote ischemic per-conditioning or post-conditioning has been shown to be neuroprotective after cerebral ischemic stroke. However, the effect of combining remote per-conditioning with post-conditioning on ischemic/reperfusion injury as well as the underlying mechanisms are largely unexplored. Methods: Here, adult male Sprague Dawley rats were subjected to middle cerebral artery occlusion (MCAO). The limb ischemic stimulus was immediately applied after onset of focal ischemia (per-conditioning), followed by repeated short episodes of remote ischemia 24 hr after reperfusion (post-conditioning). The infarct volume, motor function, and the expression of neuroglobin (Ngb) were measured at different durations after reperfusion. Results: We found that a single episode of limb remote per-conditioning afforded short-term protection, but combining repeated remote post-conditioning during the 14 days after reperfusion significantly ameliorated cerebral ischemia/reperfusion injury. Interestingly, we also found that ischemic per- and post-conditioning significantly increased expression of Ngb, an oxygen-binding globin protein that has been demonstrated to be neuroprotective against stroke, at peri-infarct regions from day 1 to day 14 following ischemia/reperfusion. Conclusion: Our results suggest that the conventional per-conditioning combined with post-conditioning may be used as a novel neuroprotective strategy against ischemia-reperfusion injury, and Ngb seems to be one of the important players in limb remote ischemia-mediated neuroprotection.