EPOXIDATION OF PLASMALOGENS - SOURCE FOR LONG-CHAIN ALPHA-HYDROXYALDEHYDES IN SUBCELLULAR-FRACTIONS OF BOVINE LIVER
EPOXIDATION OF PLASMALOGENS - SOURCE FOR LONG-CHAIN ALPHA-HYDROXYALDEHYDES IN SUBCELLULAR-FRACTIONS OF BOVINE LIVER
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DOI：
10.1042/bj3090807
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发表时间：
1995-08-01
影响因子：
4.1
通讯作者：
SPITELLER, G
中科院分区：
文献类型：
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作者：
LOIDLSTAHLHOFEN, A;HANNEMANN, K;SPITELLER, G
1. 通过应用五氟苯氧肟衍生化，隐藏的长链α -羟基醛被捕获在牛肝脏的所有亚细胞部分[van Kuijk， Thomas， Stephens和Dratz (1986) Biochem]。Biophys。Res. common . 139, 144-149]，并通过GLC/MS使用特征离子迹线进行定量。2、长链2-羟基烷烃的链长谱清楚地表明它们作为前体分子与缩醛原的关系。3. 先前假设在牛组织脂质中α -酰基羟基醛原作为掩膜长链α -羟基醛的前体分子存在[Lutz and Spiteller(1991)]。Chem. 1998, 563-567]被排除在外。4. 在所有亚细胞组分中，恒定的氧化速率为非酶性等离子体原环氧化过程（可能通过氢氧自由基）提供了确凿的证据。5. α -羟基醛的高反应性对先前假设的缩醛原保护哺乳动物细胞免受氧化应激的假设提出了一些质疑[Morand， Zoeller和Raetz(1988)]。化学学报，26,1190 - 1196；[j] .中国生物科学。化学学报，2003,19(2):387 - 398。
1. Masked long-chain alpha-hydroxyaldehydes were trapped in all subcellular fractions of bovine liver by application of pentafluorbenzyloxime derivatization [van Kuijk, Thomas, Stephens and Dratz (1986) Biochem. Biophys. Res. Commun. 139, 144-149] and quantified via GLC/MS using characteristic ion traces. 2, The chain-length profile of long-chain 2-hydroxyalkanales clearly indicates their relationship to plasmalogens as precursor molecules. 3. The previously postulated existence of alpha-acyloxyplasmalogens as precursor molecules of masked long-chain alpha-hydroxyaldehydes in bovine tissue lipids [Lutz and Spiteller (1991) Liebigs Ann. Chem. 1998, 563-567] was excluded. 4. The constant oxidation rate of plasmalogens in all subcellular fractions provides conclusive evidence for a non-enzymic plasmalogen epoxidation process (probably via hydroperoxy radicals). 5. The high reactivity of alpha-hydroxyaldehydes sheds some doubt on the postulation that plasmalogens protect mammalian cells against oxidative stress as postulated previously [Morand, Zoeller and Raetz (1988) J. Biol. Chem. 263, 11590-11596; Morand, Zoeller and Raetz (1988) J. Biol. Chem. 263, 11597-11606].