Flexibly deformable collagen hydrogel tube reproducing immunological tissue deformation of blood vessels as a pharmacokinetic testing model
Flexibly deformable collagen hydrogel tube reproducing immunological tissue deformation of blood vessels as a pharmacokinetic testing model
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柔性可变形胶原水凝胶管再现血管的免疫组织变形作为药代动力学测试模型
DOI：
10.1002/adhm.202101509
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发表时间：
2021
影响因子：
10
通讯作者：
Shun Itai and Hiroaki Onoe
中科院分区：
文献类型：
--
作者：
K. Kimura;T. Iwaya;M. Yoshida;K. Kasai;T. Hirooka;and M. Nakazawa;Shun Itai and Hiroaki Onoe
由于血管的生化反应在免疫反应或各种疾病中起着至关重要的作用，医学领域对体外血管模型有很高的要求。然而，由于缺乏组织变形的实现，血管模型往往难以忠实地模拟生物医学反应。在这里，炎症介质诱导的血管变形反应在一个灵活的可变形的胶原蛋白水凝胶管装置上被复制。一个独立的胶原蛋白管使组织在生化反应中灵活变形，并在组织和细胞水平上实现收缩。组织的收缩减轻了细胞间的压力，维持了细胞间的连接，即使紧密的连接被破坏。抗组胺药物灌注试验由于其连接部分，易于进行，可适当抑制炎症反应。分析了两种不同刚度支架对内皮细胞的牵引力约为0.9µN。研究人员认为，柔性组织模型具有复制生物化学反应的潜力，可以为生物医学研究和药代动力学测试提供高相似性的活体血管组织模型。
Since the biochemical reaction of blood vessels plays an essential role in immune response or various diseases, in vitro vascular models have high demand from medical fields. However, vascular models often tend to be difficult to mimic the biomedical reaction faithfully because of the lack of implementation of the tissue deformation. Here, an inflammatory mediator‐induced deformation reaction of a blood vessel on a flexibly deformable collagen hydrogel tube device is reproduced. A self‐standing collagen tube enables the tissue to deform flexibly in biochemical reaction and achieves contraction both at tissue and cell level. The contraction of tissue relieves the stress between cells under reaction to maintain cellular junctions even tight junctions are broken. Also, the drug perfusion test with antihistamine chemical is easily performed due to the connector part and properly inhibits the inflammatory reaction. Moreover, the traction force on endothelial cells is analyzed as about 0.9 µN on two types of scaffolds with different stiffness. It is believed that the potential of the flexible tissue model to reproduce biochemical reactions can contribute to the fabrication of vascular tissue models mimicking in vivo in high similarity as a platform for biomedical researches and pharmacokinetic testing.