A RAPID INVITRO ASSAY SYSTEM USING ANTI-BROMODEOXYURIDINE FOR DRUG SUSCEPTIBILITY OF PLASMODIUM-FALCIPARUM
A RAPID INVITRO ASSAY SYSTEM USING ANTI-BROMODEOXYURIDINE FOR DRUG SUSCEPTIBILITY OF PLASMODIUM-FALCIPARUM
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DOI：
10.1016/0035-9203(88)90404-x
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发表时间：
1988-03-01
影响因子：
2.2
通讯作者：
SHIMONO, K
中科院分区：
文献类型：
--
作者：
DOI, H;ISHII, A;SHIMONO, K
通过测量溴脱氧尿苷（BrdU）掺入寄生虫脱氧核糖核酸（DNA）来监测药物作用。用BrdU脉冲标记细胞，合成DNA的细胞将BrdU掺入其DNA中。抗BrdU用于识别脉冲时正在进行DNA合成的细胞。氯喹，乙胺嘧啶和奎宁在RPMI 1640培养基中补充BrdU培养仅3小时的浓度-效应曲线测定。用ELISA法检测恶性疟原虫对BrdU的摄取量，可区分不同的恶性疟原虫株。该检测方法为监测各种抗疟药物的效果提供了一种快速准确的方法。
Drug effects were monitored by the measurement of bromodeoxyuridine (BrdU) incorporation into parasite deoxyribonucleic acid (DNA). Cells are pulse-labelled with BrdU and those which are synthesizing DNA incorporate BrdU into their DNA. Anti-BrdU is used to identify cells undergoing DNA synthesis at the time of the pulse. Concentration-effect curves were determined for chloroquine, pyrimethamine and quinine in culture for only 3 h in RPMI 1640 medium supplemented with BrdU. Different strains ofPlasmodium falciparumcould be distinguished when BrdU uptake was monitored by the ELISA. This assay offers a fast and accurate method of monitoring the effects of a variety of antimalarial drugs.