The separation and purification of enzymes through foaming.

The separation and purification of enzymes through foaming.
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通过发泡分离纯化酶。

DOI:
10.1016/0003-2697(66)90111-4
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发表时间:
1966
影响因子:
2.9
通讯作者:
B. Paltiel
B. Paltiel
中科院分区:
生物学4区
文献类型:
--
作者:
S. Charm;J. Morningstar;C. C. Matteo;B. Paltiel

文献摘要

被引文献

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过氧化氢酶和淀粉酶的混合物通过发泡进行浓缩和纯化。考察了表面张力-浓度图与泡沫形成的关系。可采用各种药剂来控制泡沫分离过程。许多其他的酶受到泡沫被发现不灭活。结论认为,该工艺作为酶的大规模纯化和浓缩手段有待进一步研究。
Mixtures of catalase and amylase are concentrated and purified through foaming. The relationship of the surface tension-concentration diagram to foam formation is examined. Various agents may be employed to control the foam separation process. A number of other enzymes subjected to foaming are found not to be inactivated. It is concluded that this process should be investigated further as a means of large-scale purification and concentration of enzymes.