Chemical analysis and biological testing of a polar fraction of ambient air, diesel engine, and gasoline engine particulate extracts.
Chemical analysis and biological testing of a polar fraction of ambient air, diesel engine, and gasoline engine particulate extracts.
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DOI：
10.1289/ehp.94102s485
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发表时间：
1994-10
影响因子：
10.4
通讯作者：
中科院分区：
文献类型：
--
作者：
在硅胶柱上对汽、柴油汽车尾气和周围空气颗粒的萃取物按极性分成5个馏分。用正相高效液相色谱法对Ames试验中表现出高遗传毒性活性的两个中极性馏分进行进一步的减分。化学分析采用气相色谱法结合质谱法和火焰电离检测法进行。用Ames试验分析了含S9和不含S9的粗提物、馏分和亚馏分，并报道了亚馏分中最丰富的化合物。
Extracts of gasoline and diesel vehicle exhaust and ambient air particles were fractionated into five fractions according to polarity on a silica gel column. Two medium polar fractions showing high genotoxic activity in the Ames test were further subfractionated, using normal-phase high-performance liquid chromatography. Chemical analyses were performed by means of gas chromatography combined with mass spectrometry and flame ionization and detection. The crude extracts, fractions, and subfractions were assayed with the Ames test, with and without S9, and the most abundant compounds in the subfractions are reported.