DR5 as a reporter system to study auxin response in Populus
DR5 as a reporter system to study auxin response in Populus
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DOI：
10.1007/s00299-012-1378-x
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发表时间：
2013-03-01
影响因子：
6.2
通讯作者：
Busov, Victor B.
中科院分区：
文献类型：
--
作者：
Chen, Yiru;Yordanov, Yordan S.;Busov, Victor B.
生长素应答启动子DR 5报告系统在杨树中具有监测叶、根、茎等组织生长素应答的功能，我们描述了DR 5：：GUS报告系统在稳定转化的杨树植株中的行为。我们发现了几个相似之处与拟南芥，包括敏感性天然和合成生长素，快速诱导后，在各种组织中的治疗，并在根组织中的最大反应。与拟南芥也有几个重要的差异，包括更慢的最大响应时间和更低的诱导幅度。幼叶和茎节以下的顶点表现出更高的DR 5活性比老叶和茎进行二次生长。DR 5活性在皮层中最高，表明该组织中生长素浓度和/或敏感性水平高。我们的研究表明，DR 5报告系统是一个敏感和简单的系统，监测生长素的反应和分布在细胞分辨率的白杨。
Auxin responsive promoter DR5 reporter system is functional in Populus to monitor auxin response in tissues including leaves, roots, and stems.We described the behavior of the DR5::GUS reporter system in stably transformed Populus plants. We found several similarities with Arabidopsis, including sensitivity to native and synthetic auxins, rapid induction after treatment in a variety of tissues, and maximal responses in root tissues. There were also several important differences from Arabidopsis, including slower time to maximum response and lower induction amplitude. Young leaves and stem sections below the apex showed much higher DR5 activity than did older leaves and stems undergoing secondary growth. DR5 activity was highest in cortex, suggesting high levels of auxin concentration and/or sensitivity in this tissue. Our study shows that the DR5 reporter system is a sensitive and facile system for monitoring auxin responses and distribution at cellular resolution in poplar.