Overexpression of a Vesicle Trafficking Gene, OsRab7, enhances salt tolerance in rice.
Overexpression of a Vesicle Trafficking Gene, OsRab7, enhances salt tolerance in rice.
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囊泡运输基因 OsRab7 的过度表达增强了水稻的耐盐性
DOI：
10.1155/2014/483526
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发表时间：
2014
影响因子：
--
通讯作者：
Zhu Y
中科院分区：
文献类型：
--
作者：
Peng X;Ding X;Chang T;Wang Z;Liu R;Zeng X;Cai Y;Zhu Y
高土壤盐度是影响农业生产的主要因素。研究与耐盐相关基因的功能对于增强作物对压力的耐受性至关重要。 RAB7是一种小的GTP结合蛋白，在真核生物中广泛分布。由RAB7介导的内吞运输在动物和酵母细胞中起着重要作用，但是当前对植物中Rab7的理解仍然非常有限。本文中，我们与大米分离了一个囊泡运输基因Osrab7。在盐处理的条件下，过表达的Osrab7过表达的转基因大米表现出增强的幼苗生长和脯氨酸含量的增加。此外，在Osrab7转基因大米的根尖端观察到了增加的囊泡数量。 Osrab7过表达的植物表现出对盐胁迫的耐受性，这表明液泡运输对于植物的耐盐性很重要。
High soils salinity is a main factor affecting agricultural production. Studying the function of salt-tolerance-related genes is essential to enhance crop tolerance to stress. Rab7 is a small GTP-binding protein that is distributed widely among eukaryotes. Endocytic trafficking mediated by Rab7 plays an important role in animal and yeast cells, but the current understanding of Rab7 in plants is still very limited. Herein, we isolated a vesicle trafficking gene, OsRab7, from rice. Transgenic rice over-expressing OsRab7 exhibited enhanced seedling growth and increased proline content under salt-treated conditions. Moreover, an increased number of vesicles was observed in the root tip of OsRab7 transgenic rice. The OsRab7 over-expression plants showed enhanced tolerance to salt stress, suggesting that vacuolar trafficking is important for salt tolerance in plants.