Synthesis of glycopolymers with specificity for bacterial strains via bacteria-guided polymerization
Synthesis of glycopolymers with specificity for bacterial strains via bacteria-guided polymerization
复制标题
通过细菌引导聚合合成对细菌菌株具有特异性的糖聚合物
DOI：
10.1039/c8sc05561k
复制
发表时间：
2019-05-28
期刊：
影响因子：
8.4
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Luo, Yan;Gu, Yan;Chen, Hong
识别益生菌和病原体对人体的健康有很大的意义。开发具有高特异性（包括菌株特异性）的微生物菌群靶向治疗是至关重要的。在这项研究中，我们利用E。coliMG 1655细菌为活模板，高选择性地原位合成糖聚合物。利用细菌-糖单体-适配-聚合（BS-MAP）方法，我们从细菌表面获得了能识别两株大肠杆菌模板菌的糖聚合物。大肠杆菌的细菌聚集实验和QCM-D测定证实了糖聚合物的特异性细菌结合能力。此外，合成的糖聚合物在抗感染和共培养试验中显示出强大的抑制能力，可以防止细菌对细胞的伤害。
Identifying probiotics and pathogens is of great interest to the health of the human body. It is critical to develop microbiota-targeted therapies to have high specificity including strain specificity. In this study, we have utilized E. coli MG1655 bacteria as living templates to synthesize glycopolymers in situ with high selectivity. By this bacteria-sugar monomer-aptation-polymerization (BS-MAP) method, we have obtained glycopolymers from the surface of bacteria which can recognize template bacteria from two strains of E. coli and the specific bacteria-binding ability of glycopolymers was confirmed by both bacterial aggregation experiment and QCM-D measurements. Furthermore, the synthesized glycopolymers have shown a powerful inhibitory ability which can prevent bacteria from harming cells in both anti-infection and co-culture tests.