Lactate and the effects of exercise on testosterone secretion: Evidence for the involvement of a cAMP-mediated mechanism
Lactate and the effects of exercise on testosterone secretion: Evidence for the involvement of a cAMP-mediated mechanism
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DOI：
10.1097/00005768-199708000-00010
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发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Wang, PS
中科院分区：
文献类型：
--
作者：
Lu, SS;Lau, CP;Wang, PS
本实验观察了游泳和乳酸对雄性大鼠睾酮释放的影响。在体内实验中，雄性大鼠通过右颈静脉插管，并在运动后0、10、15、30和60分钟收集血液，或者它们通过右颈静脉和左股静脉插管，并在运动后0、2、5、10、15、30、60、和输注乳酸盐10分钟后120分钟（13 mg.kg（-1）.min（-1））。在游泳或水浸10分钟后，还收集了躯干血和睾丸静脉血。在一项体外实验中，用乳酸盐（0.01-10 mM）和/或人绒毛膜促性腺激素（hCG; 0.5 IU.mL（-1））激发睾丸碎片，并用乳酸盐（8 mM）激发内侧基底下丘脑（MBH）。运动后10、15和30 min血浆乳酸和睾酮水平均高于静息水平。游泳30分钟后血浆促黄体生成素（LH）升高。乳酸盐或hCG的给药以剂量依赖性方式增加睾丸环磷酸腺苷（cAMP）和睾酮释放。游泳和乳酸输注后血浆睾酮升高。MBH与乳酸的孵育增加了培养基中的促性腺激素释放激素（GnRH）水平。这些结果表明，在运动过程中，雄性大鼠血浆睾酮水平的增加至少部分是由于乳酸通过增加睾丸cAMP的产生对睾酮分泌的直接和LH-独立的刺激作用。游泳升高的血浆LH可能是乳酸引起的GnRH升高的结果。
The effects of swimming and lactate on the release of testosterone were examined in male rats. During in vivo experiments, male rats were catheterized via the right jugular vein and blood was collected at 0, 10, 15, 30, and 60 min following the exercise, or they were catheterized via the right jugular vein and the left femoral vein and blood was collected at 0, 2, 5, 10, 15, 30, 60, and 120 min after a 10-min infusion of lactate (13 mg.kg(-1).min(-1)). Trunk blood and blood from the testicular vein were also collected after 10 min of swimming or water immersion. In an in vitro experiment, testicular fragments were challenged with lactate (0.01-10 mM) and/or human chorionic gonadotropin (hCG; 0.5 IU.mL(-1)), and the mediobasal hypothalamus (MBH) was challenged with lactate (8 mM). The postexercise levels of plasma lactate and testosterone at 10, 15, and 30 min were higher than resting levels. Plasma luteinizing hormone (LH) was increased following 30 min of swimming. Administration of lactate or hCG increased in a dose dependent manner testicular cyclic adenosine 3':5' monophosphate (cAMP) and testosterone release. Plasma testosterone increased after swimming and lactate infusion. Incubation of MBH with lactate increased the gonadotropin-releasing hormone (GnRH) level in the medium. These results suggest that the increased plasma testosterone levels in male rats during exercise is at least partially a result of a direct and LH-independent stimulatory effect of lactate on the secretion of testosterone by increasing testicular cAMP production. Swim-elevated plasma LH may be a result of a rise of GnRH caused by lactate.