Expressed genes in regenerating rat liver after partial hepatectomy.
Expressed genes in regenerating rat liver after partial hepatectomy.
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DOI：
10.3748/wjg.v11.i19.2932
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发表时间：
2005-05
影响因子：
4.3
通讯作者：
Cunshuan Xu;Cuifang Chang;Jinyun Yuan;Wenqiang Li;H. Han;Kejin Yang;Lifeng Zhao;Yuchang Li;Hui-yong Zhang;S. Rahman;Jingbo Zhang
中科院分区：
文献类型：
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作者：
Cunshuan Xu;Cuifang Chang;Jinyun Yuan;Wenqiang Li;H. Han;Kejin Yang;Lifeng Zhao;Yuchang Li;Hui-yong Zhang;S. Rahman;Jingbo Zhang
目的研究肝部分切除(PH)后大鼠再生肝组织中551个基因的表达谱，揭示肝再生及其调控机制和肝病的发生机制。方法将抑制消减杂交筛选出的551个表达序列标签制成内部基因芯片，利用基因芯片和生物信息学方法分析再生大鼠肝脏中的表达基因及其表达谱。结果在分析的551个基因中，有300个基因在LR期间的一个或多个时间点上调或下调2倍以上。大多数基因表达上调或下调2-5倍，但最高可达对照的90倍。其中139个基因表达上调，135个基因表达下调，26个基因的表达上调或下调显示对再生肝的依赖。在24小时再生肝中表达的基因明显多于其他各组。聚类分析和泛化分析表明，基因在再生肝脏中的表达至少有6种不同的时间模式，即基因在即刻早期、早期、中期、早-晚期、晚期、末期表达。结论在LR中，下调基因的数量与上调基因的数量基本一致，连续变化的基因多于快速瞬变基因。PH后2小时和4小时、12小时和16小时、48小时和96小时、72小时和144小时基因表达的时间模式相似。基因芯片结合抑制性消减杂交技术可以有效地筛选与LR相关的基因。
AIM To reveal the liver regeneration (LR) and its control as well as the occurrence of liver disease and to study the gene expression profiles of 551 genes after partial hepatectomy (PH) in regenerating rat livers. METHODS Five hundred and fifty-one expressed sequence tags screened by suppression subtractive hybridization were made into an in-house cDNA microarray, and the expressive genes and their expressive profiles in regenerating rat livers were analyzed by microarray and bioinformatics. RESULTS Three hundred of the analyzed 551 genes were up- or downregulated more than twofolds at one or more time points during LR. Most of the genes were up- or downregulated 2-5 folds, but the highest reached 90 folds of the control. One hundred and thirty-nine of them showed upregulation, 135 displayed downregulation, and up or down expression of 26 genes revealed a dependence on regenerating livers. The genes expressed in 24-h regenerating livers were much more than those in the others. Cluster analysis and generalization analysis showed that there were at least six distinct temporal patterns of gene expression in the regenerating livers, that is, genes were expressed in the immediate early phase, early phase, intermediate phase, early-late phase, late phase, terminal phase. CONCLUSION In LR, the number of down-regulated genes was almost similar to that of the upregulated genes; the successively altered genes were more than the rapidly transient genes. The temporal patterns of gene expression were similar 2 and 4 h, 12 and 16 h, 48 and 96 h, 72 and 144 h after PH. Microarray combined with suppressive subtractive hybridization can effectively identify the genes related to LR.