The properties of the chlorophyll a/b-binding proteins Lhca2 and Lhca3 studied in vivo using antisense inhibition
The properties of the chlorophyll a/b-binding proteins Lhca2 and Lhca3 studied in vivo using antisense inhibition
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DOI：
10.1104/pp.127.1.150
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发表时间：
2001-09-01
期刊：
影响因子：
7.4
通讯作者：
Jansson, S
中科院分区：
文献类型：
--
作者：
Ganeteg, U;Strand, Å;Jansson, S
研究了捕光蛋白Lhca 2和Lhca 3在拟南芥哥伦比亚型反义植物中的特异功能，其中蛋白质被单独抑制。反义效应在每种植物中是特异性的，但是除了靶向产物之外的Lhca蛋白的水平也受到影响。Lhca 1和Lhca 4的含量不受影响，但Lhca 3（在Lhca 2-阻遏植物）几乎完全耗尽，Lhca 2下降到约30%的野生型水平在Lhca 3-阻遏植物。这表明Lhca 2和Lhca 3蛋白彼此物理接触，并且它们需要彼此的稳定性。光系统I在730 nm处的荧光被认为是从与Lhca 1和Lhca 4结合的色素发出的。然而，荧光发射和激发光谱表明，Lhca 2和Lhca 3，在体外荧光在680 nm，也可以有助于远红荧光在体内。在695和715 nm处具有最大吸收的光谱形式，显然分别在702和735 nm处具有最大发射，可能与Lhca 2和Lhca 3相关。
The specific functions of the light-harvesting proteins Lhca2 and Lhca3 were studied in Arabidopsis ecotype Colombia antisense plants in which the proteins were individually repressed. The antisense effect was specific in each plant, but levels of Lhca proteins other than the targeted products were also affected. The contents of Lhca1 and Lhca4 were unaffected, but Lhca3 (in Lhca2-repressed plants) was almost completely depleted, and Lhca2 decreased to about 30% of wild-type levels in Lhca3-repressed plants. This suggests that the Lhca2 and Lhca3 proteins are in physical contact with each other and that they require each other for stability. Photosystem I fluorescence at 730 nm is thought to emanate from pigments bound to Lhca1 and Lhca4. However, fluorescence emission and excitation spectra suggest that Lhca2 and Lhca3, which fluoresce in vitro at 680 run, also could contribute to far-red fluorescence in vivo. Spectral forms with absorption maxima at 695 and 715 run, apparently with emission maxima at 702 and 735 nm, respectively, might be associated with Lhca2 and Lhca3.