Sequencing and analysis of the complete mitochondrial genome of tundra shrew (Sorex tundrensis) from China
Sequencing and analysis of the complete mitochondrial genome of tundra shrew (Sorex tundrensis) from China
复制标题
DOI：
10.3109/19401736.2015.1025261
复制
发表时间：
2016-01-01
影响因子：
--
通讯作者：
Dou, Huashan
中科院分区：
文献类型：
--
作者：
Xu, Chunzhu;Zhao, Shuai;Dou, Huashan
利用长片段PCR技术测定了中国苔原鼩（Sorex tundrensis）的丝裂基因组全序列。基因组全长17, 444 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、23个转运RNA基因、1个L链复制起点和1个控制区。重链的总碱基组成为A（32.9%）、C（24.8%）、T（29.0%）和G（13.3%）。碱基组成存在明显的A-T偏斜，这种偏斜在控制区和蛋白质编码基因中最为明显。在苔原鼩线粒体基因组控制区中定义了延伸的终止相关序列结构域、中央保守结构域和保守的序列块结构域。基于MP、ML、NJ和贝叶斯分析的线粒体基因组分析产生了相同的系统发育树。三种Sorex形成了一个单系群，在所有的检查中具有高的自举值（100%）。
The complete mitogenome sequence of tundra shrew (Sorex tundrensis) was determined using long PCR. The genome was 17,444 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 23 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (32.9%), C (24.8%), T (29.0%), and G (13.3%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. The extended termination-associated sequence domain, the central conserved domain and the conserved sequence block domain are defined in the mitochondrial genome control region of tundra shrew. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. The three Sorex species formed a monophyletic group with the high bootstrap value (100 %) in all examinations.