Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.
Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.
复制标题
通过连续固相和液-液萃取对血浆总 15 系列 F(2)-异前列腺素进行定量。
DOI：
10.1016/j.ab.2009.09.042
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发表时间：
2010
影响因子：
2.9
通讯作者：
Traber,MaretG
中科院分区：
文献类型：
--
作者：
Taylor,AlanW;Traber,MaretG
F2-异前列腺素是前列腺素F2α(PgF2α)的立体异构体和区域异构体，可作为脂质过氧化的生物标志物。我们改进了液-液萃取(LLE)程序，用于F2-异前列腺素分析(ANAL。生物化学。350(2006)41-51)，使用固相萃取(SPE)和LLE相结合的方法来生产更清洁、易于浓缩的萃取物。此外，缩短高效液相色谱(HPLC)分离时间增加了高效液相-串联质谱仪(LC-MS/MS)分析中的峰高。这两个变化都提高了检测的总体灵敏度。MS/MS分析用于确认特定峰的身份，这些峰可能是比通常测量的8-iso-PGF2α更好的生物标志物。
F2-isoprostanes are stereo- and regioisomers of prostaglandin F2α(PGF2α) and are used as biomarkers for lipid peroxidation. We modified our liquid–liquid extraction (LLE) procedure for F2-isoprostane analysis (Anal. Biochem. 350 (2006) 41–51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC–tandem mass spectrometry (HPLC–MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF2α.